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リコンビナントRabMAb

Anti-LIFR 抗体 [EPR24865-56] (BSA and Azide free) (ab300552)

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Western blot - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
  • Western blot - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
  • Immunoprecipitation - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
  • Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24865-56] to LIFR - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-LIFR antibody [EPR24865-56] (BSA and Azide free)
    LIFR 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24865-56] to LIFR - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HeLa, T-47D, MDA-MB-231 whole cell lysates; HepG2 lysate treated with Protein Deglycosylation Mix II. IP; HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.

  • 特記事項

    ab300552 is the carrier-free version of ab300551.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300552の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.

  • 関連疾患

    Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS) [MIM:601559]; also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue.
    Note=A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1.

  • 配列類似性

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 6 fibronectin type-III domains.

  • ドメイン

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD118 antibody
      • CD118 antigen antibody
      • FLJ98106 antibody
      • FLJ99923 antibody
      • Leukemia inhibitory factor receptor alpha antibody
      • Leukemia inhibitory factor receptor antibody
      • LIF R antibody
      • LIF receptor antibody
      • LIF-R antibody
      • Lifr antibody
      • LIFR_HUMAN antibody
      • SJS2 antibody
      • STWS antibody
      • SWS antibody
      see all

    画像

    • Western blot - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
      Western blot - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
      Lane 1 : Anti-LIFR antibody [EPR24865-56] (ab300551) at 1000 µg
      Lane 2 : Anti-LIFR antibody [EPR24865-56] (ab300551) at 1/1000 dilution

      Lane 1 : Untreated HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate
      Lane 2 : HepG2 lysate treated with Protein Deglycosylation Mix II

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 120,180 kDa why is the actual band size different from the predicted?



      This data was developed using ab300551, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      LIFR is a glycoprotein of approximately 180 kDa and detected as a 120 kDa band after treated with Protein Deglycosylation MIX II.

      Exposure time: 3 minutes

    • Western blot - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
      Western blot - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
      All lanes : Anti-LIFR antibody [EPR24865-56] (ab300551) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate
      Lane 3 : T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
      Lane 4 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 180 kDa why is the actual band size different from the predicted?



      This data was developed using ab300551, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: Negative control: T-47D (PMID: 23001183)

      Exposure time: 59 seconds

    • Immunoprecipitation - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
      Immunoprecipitation - Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)

      This data was developed using ab300551, the same antibody clone in a different buffer formulation.

      LIFR was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab300551 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300551 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg

      Lane 2: ab300551 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300551 in HeLa whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 145 seconds

      The bands beneath the target band in lane 2 are expected to be degradation products.

    • Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)
      Anti-LIFR antibody [EPR24865-56] (BSA and Azide free) (AB300552)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300552 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300552 は論文での使用が確認できていません。

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