Anti-LIFR 抗体 [EPR24865-56] (ab300551)
![Western blot - Anti-LIFR antibody [EPR24865-56] (AB300551)](https://www.abcam.co.jp/ps/products/300/ab300551/Images/AB300551-487620-anti-lifr-antibody-epr2486556-western-blot-untreated-hepg2-human-hepatocellular-carcinoma-epithelial-cell-whole-cell-lysate20-ug.jpg "Western blot - Anti-LIFR antibody [EPR24865-56] (AB300551)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24865-56] to LIFR
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LIFR antibody [EPR24865-56]
LIFR 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24865-56] to LIFR -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt (Intra),ICC/IF or IHC-P -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa, HeLa, T-47D, MDA-MB-231 whole cell lysates; HepG2 lysate treated with Protein Deglycosylation Mix II. IP; HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24865-56 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300551の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000.
|
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| IP |
1/30.
|
| 特記事項 |
|---|
|
WB
1/1000. |
|
IP
1/30. |
ターゲット情報
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機能
Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells. -
関連疾患
Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS) [MIM:601559]; also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue.
Note=A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 6 fibronectin type-III domains. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3977 Human
- Omim: 151443 Human
- SwissProt: P42702 Human
- Unigene: 133421 Human
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別名
- CD118 antibody
- CD118 antigen antibody
- FLJ98106 antibody
see all
画像
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Western blot - Anti-LIFR antibody [EPR24865-56] (AB300551)All lanes : Anti-LIFR antibody [EPR24865-56] (ab300551) at 1/1000 dilution
Lane 1 : Untreated HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate
Lane 2 : HepG2 lysate treated with Protein Deglycosylation Mix II
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 120,180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
LIFR is a glycoprotein of approximately 180 kDa and detected as a 120 kDa band after treated with Protein Deglycosylation MIX II.
Exposure time: 3 minutes
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![Western blot - Anti-LIFR antibody [EPR24865-56] (AB300551)](https://www.abcam.co.jp/ps/products/300/ab300551/Images/AB300551-487619-anti-lifr-antibody-epr2486556-western-blot-hela-human-cervix-adenocarcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-LIFR antibody [EPR24865-56] (AB300551)All lanes : Anti-LIFR antibody [EPR24865-56] (ab300551) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate
Lane 3 : T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
Lane 4 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: T-47D (PMID: 23001183)
Exposure time: 59 seconds
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![Immunoprecipitation - Anti-LIFR antibody [EPR24865-56] (AB300551)](https://www.abcam.co.jp/ps/products/300/ab300551/Images/AB300551-2-anti-lifr-antibody-epr2486556-immunoprecipitation-hela.jpg)
Immunoprecipitation - Anti-LIFR antibody [EPR24865-56] (AB300551)LIFR was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab300551 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300551 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab300551 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300551 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 145 seconds
The bands beneath the target band in lane 2 are expected to be degradation products.
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![Anti-LIFR antibody [EPR24865-56] (AB300551)](https://www.abcam.co.jp/ps/products/300/ab300551/Images/AB300551-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-LIFR antibody [EPR24865-56] (AB300551)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300551 は論文での使用が確認できていません。