Anti-LIF 抗体 [EPR28693-84]

• WB

Lab

Western blot - Anti-LIF antibody [EPR28693-84] (AB320821)

Western blot : Rabbit Monoclonal [EPR28693-84] to LIF ab320821 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 65-80 kDa. The identity of the band detected at ~30kDa is unknown. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LIF antibody [EPR28693-84] (ab320821) at 1/1000 dilution

Lane 1:

THP-1 at 20 µg

Lane 2:

PANC-1 at 20 µg

Lane 3:

PANC-1 Supernatant at 15 µL

Lane 4:

BT-549 at 20 µg

Lane 5:

BT-549 Supernatant at 15 µL

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 65-90 kDa,37 kDa

false

• WB

Supplier Data

Western blot - Anti-LIF antibody [EPR28693-84] (AB320821)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1-2 : 48 seconds; lane3-4 : 180 seconds

All lanes:

Western blot - Anti-LIF antibody [EPR28693-84] (ab320821) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 40 µg

Lane 2:

Human skeletal muscle tissue lysate at 40 µg

Lane 3:

Mouse spleen tissue lysate at 40 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-LIF antibody [EPR28693-84] (AB320821)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The up-regulation of LIF is induced by TNF alpha treatment (PMID : 8895217).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lane 1-2 : 7 seconds, lane 3-4 : 70 seconds

All lanes:

Western blot - Anti-LIF antibody [EPR28693-84] (ab320821) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 treated with 25ng/ml TNF alpha for 24 hours whole cell lysate at 20 µg

Lane 3:

Untreated PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 4:

PC-3 treated with 50ng/ml TNF alpha and 300ng/ml BFA for 16 hours, whole cell lysate at 40 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 3 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa,124 kDa

false

• WB

Supplier Data

Western blot - Anti-LIF antibody [EPR28693-84] (AB320821)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LIF antibody [EPR28693-84] (ab320821) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Lane 2:

NIH/3T3 treated with 300ng/ml BFA for 24 hours whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa,124 kDa

false

Exposure time: 15s