Anti-LI Cadherin 抗体 [EPR3996] (ab109190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3996] to LI Cadherin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-LI Cadherin antibody [EPR3996]
LI Cadherin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3996] to LI Cadherin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human small intestine, Human colon cancer, and HT-29 cell lysates. Human LoVo, small intestine and colon lysates. IHC-P: Human colon and Human colonic adenocarcinoma tissues
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 2.90 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3996 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109190の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport. -
組織特異性
Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin. -
配列類似性
Contains 7 cadherin domains. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1015 Human
- Omim: 603017 Human
- SwissProt: Q12864 Human
- Unigene: 591853 Human
-
別名
- BILL-cadherin antibody
- CAD17_HUMAN antibody
- Cadherin 16 antibody
see all
画像
-
Lanes 1-3 : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution (Purified)
Lanes 4-5 : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution
Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Human small intestine lysate
Lane 3 : Human colon lysate
Lane 4 : Human spleen lysate
Lane 5 : Human kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 18552820).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling LI Cadherin with purified ab109190 at 1/300 dilution (0.48 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemical staining of paraffin-embedded Human colon tissue using ab109190 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution
Lane 1 : Human small intestine cell lysate
Lane 2 : Human colon cancer cell lysate
Lane 3 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 92 kDa -
Immunohistochemical staining of paraffin-embedded Human colonic adenocarcinoma tissue using ab109190 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing negative staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing positive staining in Normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab109190 は論文での使用が確認できていません。