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AB109594

Anti-LGN 抗体

Anti-LGN antibody

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(1 Publication)

Rabbit Polyclonal LGN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GPSM2 aa 600 to C-terminus.

別名を表示する

LGN, GPSM2, G-protein-signaling modulator 2, Mosaic protein LGN

2 Images
Immunoprecipitation - Anti-LGN antibody (AB109594)
  • IP

Unknown

Immunoprecipitation - Anti-LGN antibody (AB109594)

Detection of LGN by Western Blot of Immunprecipitate.
ab109594 at 1μg/ml staining LGN in HeLa whole cell lysate immunoprecipitated using ab109594 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-LGN antibody (ab109594)

Predicted band size: 77 kDa

false

Western blot - Anti-LGN antibody (AB109594)
  • WB

Unknown

Western blot - Anti-LGN antibody (AB109594)

All lanes:

Western blot - Anti-LGN antibody (ab109594) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 77 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human GPSM2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P81274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2.00000-5.00000 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000.00000 - 1/10000.00000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LGN also known as GPSM2 (G-protein signaling modulator 2) is a protein involved in cellular processes related to signaling and cell polarity. It has a molecular mass of approximately 80 kDa. LGN is expressed in various tissues including the central nervous system where it plays a role in neural cell functions. This protein acts mechanically by interacting with G-proteins and aiding in proper signal transduction.
Biological function summary

The protein LGN influences cell division by regulating spindle orientation which is essential for accurate cell fate determination. LGN is a part of a complex with the proteins NuMA (nuclear mitotic apparatus protein) and dynein-dynactin which assists in the positioning of the mitotic spindle during cellular division. This activity ensures that cells divide correctly maintaining tissue organization and function.

Pathways

LGN is important in asymmetric cell division and cellular signaling. It is involved in the Wnt signaling pathway which is known for its role in cell growth and development. Additionally LGN interacts with G-proteins modulating their activity to facilitate proper signal transduction within these pathways. The influences on these pathways highlight the protein's necessity in maintaining cellular organization and signaling fidelity.

LGN is linked to certain neurodevelopmental disorders such as microcephaly where patients exhibit reduced brain size due to abnormal cell division. It also connects to certain types of cancer through its role in maintaining correct spindle orientation and polarity which is important for preventing uncontrolled cell proliferation. LGN's interaction with proteins like NuMA and G-proteins remains a focal point in understanding its impact on these conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays an important role in mitotic spindle pole organization via its interaction with NUMA1 (PubMed : 11781568, PubMed : 15632202, PubMed : 21816348). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed : 22327364). Plays a role in metaphase spindle orientation (PubMed : 22327364). Also plays an important role in asymmetric cell divisions (PubMed : 21816348). Has guanine nucleotide dissociation inhibitor (GDI) activity towards G(i) alpha proteins, such as GNAI1 and GNAI3, and thereby regulates their activity (By similarity).
See full target information GPSM2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 20:e47097 PubMed31321879

2019

Proteostasis is essential during cochlear development for neuron survival and hair cell polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen Freeman,Susana Mateo Sánchez,Ronald Pouyo,Pierre-Bernard Van Lerberghe,Kevin Hanon,Nicolas Thelen,Marc Thiry,Giovanni Morelli,Laura Van Hees,Sophie Laguesse,Alain Chariot,Laurent Nguyen,Laurence Delacroix,Brigitte Malgrange
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

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