Anti-LexA DNA Binding Region 抗体 (ab174384)
Key features and details
- Rabbit polyclonal to LexA DNA Binding Region
- Suitable for: WB, IP, ChIP, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG
製品の概要
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製品名
Anti-LexA DNA Binding Region antibody
LexA DNA Binding Region 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LexA DNA Binding Region -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ChIP, ICC/IFmore details -
種交差性
交差種: Escherichia coli -
免疫原
Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region.
Database link: POA7C2 -
ポジティブ･コントロール
- E. coli whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174384の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/3000. Predicted molecular weight: 22 kDa.
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| IP |
Use at an assay dependent concentration.
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| ChIP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/3000. Predicted molecular weight: 22 kDa. |
|
IP
Use at an assay dependent concentration. |
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ChIP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 1039346 Escherichia coli
- Entrez Gene: 948544 Escherichia coli
- Entrez Gene: 960080 Escherichia coli
- SwissProt: P0A7C2 Escherichia coli
- SwissProt: P0A7C3 Escherichia coli
- SwissProt: P0A7C4 Escherichia coli
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別名
- ExrA antibody
- Lex A antibody
- LexA antibody
see all
画像
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Western blot - Anti-LexA DNA Binding Region antibody (ab174384)All lanes : Anti-LexA DNA Binding Region antibody (ab174384) at 1/1000 dilution
Lane 1 : Marker
Lane 2 : E. coli whole cell lysate
Predicted band size: 22 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab174384 は 4 報の論文で使用されています。
- Hughes KJ et al. An RNA Repair Operon Regulated by Damaged tRNAs. Cell Rep 33:108527 (2020). PubMed: 33357439
- Kramarz K et al. The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal. Nat Commun 11:5643 (2020). PubMed: 33159083
- Sun Y et al. Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria. Front Cell Infect Microbiol 10:592906 (2020). PubMed: 33569353
- Hishida T et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004). WB ; Escherichia coli . PubMed: 15289460
