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AB248355

Anti-Leukotriene B4 Receptor/BLT 抗体 [EPR7113] - BSA and Azide free

Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free

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Rabbit Recombinant Monoclonal Leukotriene B4 Receptor/BLT antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

別名を表示する

BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7, LTB4R, Leukotriene B4 receptor 1, LTB4-R 1, LTB4-R1, Chemoattractant receptor-like 1, G-protein coupled receptor 16, P2Y purinoceptor 7, P2Y7

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

This data was developed using ab131041, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

This data was developed using ab131041, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)
  • WB

Unknown

Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

This data was developed using ab131041, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (<a href='/products/primary-antibodies/leukotriene-b4-receptor-blt-antibody-epr7113-ab131041'>ab131041</a>) at 1/10000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

Human fetal lung tissue lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

false

OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR7113

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

ab248355 is the carrier-free version of ab131041.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Leukotriene B4 Receptor often referred to as BLT or BLT1 plays a significant mechanical role in the immune response by binding to leukotriene B4 (LTB4) a potent chemoattractant. This receptor has a molecular mass of about 45 kDa. It expresses primarily on leukocytes including neutrophils macrophages and certain T cells. Furthermore BLT1 can also be observed in other tissues such as the spleen and liver where immune modulation has essential roles.
Biological function summary

The function of BLT1 involves mediating cell migration particularly during inflammatory responses. When LTB4 binds to BLT1 it activates intracellular signaling pathways that lead to chemotaxis and activation of immune cells. This receptor does not function in isolation; it forms part of receptor complexes on the cell surface to facilitate effective signal transduction. Although its name suggests specificity BLT1 can interact with other related lipid mediators amplifying its role in inflammation.

Pathways

Leukotriene B4 receptor BLT1 is integral to the arachidonic acid pathway and the GPCR signaling pathway. In the arachidonic acid pathway BLT1 contributes to the production of inflammatory mediators that recruit immune cells to sites of injury or infection. This receptor shares pathways with other GPCRs facilitating coordinated responses to external stimuli. It is closely related to protein kinase C (PKC) in the pathways which plays a role in signal transduction and cellular communication.

Leukotriene B4 receptor BLT1 has significant implications in chronic inflammatory diseases such as asthma and rheumatoid arthritis. Overactivation of BLT1 can result in excessive recruitment of immune cells exacerbating inflammation and tissue damage in these conditions. The receptor is also connected to COX-2 which is involved in the inflammation process linking BLT1 to the broader picture of inflammatory pathways in disease states. Understanding BLT1's role in these diseases helps identify potential therapeutic targets for managing inflammation-related pathologies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
See full target information LTB4R
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Abcam product promise

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