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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Leukotriene A4 hydrolase/LTA4H 抗体 [EPR5712] (ab109434)

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Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
  • Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
  • OI-RD Scanning - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
  • Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5712] to Leukotriene A4 hydrolase/LTA4H
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712]
    Leukotriene A4 hydrolase/LTA4H 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5712] to Leukotriene A4 hydrolase/LTA4H

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, 293T, T47-D, and A549 cell lysates. IHC-P: Human breast carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109434の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 69 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 69 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

画像

  • Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
    Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
    All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : LTA4H knockout HEK293T cell lysate
    Lane 3 : T-47D cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab109434 observed at 69 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab109434 Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] was shown to specifically react with Leukotriene in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266467 (knockout cell lysate ab258034) was used. Wild-type and Leukotriene knockout samples were subjected to SDS-PAGE. ab109434 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)

    Immunohistochemical analysis of Leukotriene A4 hydrolase/LTA4H in paraffin-embedded Human breast carcinoma tissue using ab109434 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
    Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
    All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : T47D cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 69 kDa

  • OI-RD Scanning - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
    OI-RD Scanning - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)
    Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434)

参考文献 (0)

ab109434 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109434 は論文での使用が確認できていません。

レビューと Q&A

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Question

Product code: 133512

Inquiry: Please can you clarify the difference between ab133512 and ab109434. I wish to quantify Leukotriene A4 hydrolase in human leukocytes by western blot. Which of these two products suits this application better.

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Answer

Thank you for contacting us.

Both antibodies are raised in rabbit but they are from two different clones (ab109434 from clone EPR5712 compared to ab133512 from clone EPR5713).

There are slight variations between the two products such as the immunogen sequence, which is amino acids 35-60 for ab109434 and amino acids 575-600 for ab133512 of Human Leukotriene A4 hydrolase.

Furthermore the products are tested in different species and applications. Antibody ab109434 reacts with rat and human but does not react with mouse and is suitable for WB, IP, IHC-P as well as Flow Cyt. Compared to that antibody ab133512 reacts with mouse, rat and human and was tested in WB, IHC-P, ICC/IF and Flow Cyt applications.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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