Anti-Lethal factor 抗体 [LF2]

ターゲットの情報

Lethal factor (LF), which constitutes one of the three proteins composing the anthrax toxin, is able to trigger rapid cell death in macrophages (PubMed : 10475971, PubMed : 11104681, PubMed : 3711080, PubMed : 8380282, PubMed : 9563949, PubMed : 9703991). Acts as a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5) : cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes (PubMed : 10475971, PubMed : 11104681, PubMed : 14718925, PubMed : 9563949, PubMed : 9703991). Also cleaves mouse Nlrp1b : host Nlrp1b cleavage promotes ubiquitination and degradation of the N-terminal part of Nlrp1b by the proteasome, thereby releasing the cleaved C-terminal part of Nlrp1b, which polymerizes and forms the Nlrp1b inflammasome followed by host cell pyroptosis (PubMed : 10338520, PubMed : 19651869, PubMed : 30872531, PubMed : 31268597). Able to cleave mouse Nlrp1b alleles 1 and 5, while it is not able to cleave Nlrp1b alleles 2, 3 and 4 (PubMed : 16429160, PubMed : 19651869). In contrast, does not cleave NLRP1 human ortholog (PubMed : 19651869). LF is not toxic by itself and only acts as a lethal factor when associated with protective antigen (PA) to form the lethal toxin (LeTx) : PA is required for LF translocation into the host cytosol (PubMed : 10475971, PubMed : 11104681, PubMed : 9563949, PubMed : 9703991).