Anti-Leptin 抗体 (ab16227)
Key features and details
- Rabbit polyclonal to Leptin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Leptin antibody
Leptin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Leptin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment
交差が予測される動物種: Goat, Horse, Cow, Cat, Dog, Chimpanzee, Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human Leptin aa 91-106.
Sequence:SRNVIQISNDLENLRD
Database link: P41159 -
ポジティブ・コントロール
- Human Serum
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16227の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/20 - 1/200.
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WB | (1) |
1/500 - 1/5000. Detects a band of approximately 16 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
|
特記事項 |
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ICC/IF
1/20 - 1/200. |
WB
1/500 - 1/5000. Detects a band of approximately 16 kDa. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
関連疾患
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
配列類似性
Belongs to the leptin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 493838 Cat
- Entrez Gene: 449638 Chimpanzee
- Entrez Gene: 280836 Cow
- Entrez Gene: 3952 Human
- Entrez Gene: 16846 Mouse
- Omim: 164160 Human
- SwissProt: Q9N2C1 Cat
- SwissProt: O02750 Chimpanzee
see all -
別名
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
画像
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Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin antibody (ab16227)Ab16227 staining human placenta.
Left panel: with primary antibody. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
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Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (31)
ab16227 は 31 報の論文で使用されています。
- Song Q et al. Nonmetastatic Axillary Lymph Nodes Have Distinct Morphology and Immunophenotype in Obese Patients with Breast Cancer at Risk for Metastasis. Am J Pathol 194:253-263 (2024). PubMed: 38029922
- Yamanaka Y et al. Adiponectin inhibits fibrosis of the palmar aponeurosis in Dupuytren's contracture in male patients. Bone Joint Res 12:486-493 (2023). PubMed: 37536684
- McCarty KJ et al. Effects of Exogenous Glucocorticoid Infusion on Appetitic Center Development in Postnatal Dairy Bull Calves. Animals (Basel) 13:N/A (2023). PubMed: 37370490
- Li J et al. Acupuncture improves immunity and fatigue after chemotherapy in breast cancer patients by inhibiting the Leptin/AMPK signaling pathway. Support Care Cancer 31:506 (2023). PubMed: 37542585
- Ren G et al. Berberine inhibits carcinogenesis through antagonizing the ATX-LPA-LPAR2-p38-leptin axis in a mouse hepatoma model. Mol Ther Oncolytics 26:372-386 (2022). PubMed: 36090480