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リコンビナントRabMAb

Anti-LEPRE1/P3H1 抗体 [EPR10193(B)] (ab154799)

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Indirect ELISA - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
  • Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
  • Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
  • Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
  • Flow Cytometry (Intracellular) - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
  • Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, Indirect ELISA
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-LEPRE1/P3H1 antibody [EPR10193(B)]
    LEPRE1/P3H1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, Indirect ELISAmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human LEPRE1/P3H1. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • HeLa, HepG2, A549 and Human placenta lysates; HepG2 and A549 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154799の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
ICC/IF
1/250 - 1/500.
Indirect ELISA
Use at an assay dependent concentration.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
ICC/IF
1/250 - 1/500.
Indirect ELISA
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.

  • 関連疾患

    Defects in LEPRE1 are the cause of osteogenesis imperfecta type 8 (OI8) [MIM:610915]. A connective tissue disorder characterized by disproportionate short stature, severe osteoporosis, shortening of the long bones, white sclerae, a round face and a short barrel-shaped chest.

  • 配列類似性

    Belongs to the leprecan family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 4 TPR repeats.

  • 翻訳後修飾

    O-glycosylated; chondroitin sulfate.

  • 細胞内局在

    Endoplasmic reticulum. Secreted > extracellular space > extracellular matrix. Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan.
  • Information by UniProt

  • 参照データベース

    • 別名

      • GROS 1 antibody
      • GROS1 antibody
      • Growth suppressor 1 antibody
      • LEPRE 1 antibody
      • Lepre1 antibody
      • Leprecan 1 antibody
      • Leprecan antibody
      • Leprecan-1 antibody
      • Leprecan1 antibody
      • Leucine proline enriched proteoglycan (leprecan) 1 antibody
      • Leucine proline enriched proteoglycan 1 antibody
      • Leucine- and proline-enriched proteoglycan 1 antibody
      • MGC117314 antibody
      • OI8 antibody
      • P3H1 antibody
      • P3H1_HUMAN antibody
      • Prolyl 3 hydroxylase 1 antibody
      • Prolyl 3-hydroxylase 1 antibody
      see all

    画像

    • Indirect ELISA - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
      Indirect ELISA - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

      ELISA using ab154799 at varying antibody concentrations and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

    • Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
      Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
      All lanes : Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Human placenta tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 83 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
      Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling LEPRE1/P3H1 with Purified ab154799 at 1/500 dilution (5µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
      Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

      Immunofluorescence analysis of HepG2 cells labeling LEPRE1/P3H1 with ab154799 at 1/250 dilution.

    • Flow Cytometry (Intracellular) - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
      Flow Cytometry (Intracellular) - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

      Intracellular flow cytometric analysis of permeabilized A549 cells labeling LEPRE1/P3H1 with ab154799 at 1/10 dilution (red) or a negative rabbit IgG (green). 

       

       

    • Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
      Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

    参考文献 (1)

    ab154799 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154799 は 1 報の論文で使用されています。

    • Wu J  et al. Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations. Cell Mol Life Sci N/A:N/A (2019). PubMed: 30993352

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-LEPRE1/P3H1 antibody [EPR10193(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT29)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Treatment
    shRNA against LEPRE1
    Specification
    HT29
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Jun 03 2016

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