Anti-Leishmania A2 抗体 [C9] (ab150344)
Key features and details
- Mouse monoclonal [C9] to Leishmania A2
- Suitable for: WB, IP, ICC/IF
- Reacts with: Leishmania
- Isotype: IgG1
製品の概要
-
製品名
Anti-Leishmania A2 antibody [C9] -
製品の詳細
Mouse monoclonal [C9] to Leishmania A2 -
由来種
Mouse -
特異性
A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence is particular to the parasite and therefore the antibody has no cross-reactivity with mammalian proteins. It is specific to A2 protein from Leishmania donovani and in a highly probable manner to other species which express A2. It won't be detected in species where the A2 is a non-expressed pseudogene such as Leishmania major.
ab150344 recognizes the family of A2 proteins ranging from 42 to 100kDa.
-
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Leishmania -
免疫原
The details of the immunogen for this antibody are not available.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 99% PBS
Contains no additives. -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Lyophilized protein G purified in PBS pH7.4 -
ポリ/モノ
モノクローナル -
クローン名
C9 -
アイソタイプ
IgG1
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150344の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/100.
|
|
| IP |
Use at an assay dependent dilution.
|
|
| ICC/IF |
Use at an assay dependent dilution.
|
| 特記事項 |
|---|
|
WB
1/100. |
|
IP
Use at an assay dependent dilution. |
|
ICC/IF
Use at an assay dependent dilution. |
ターゲット情報
-
関連性
Leishmania donovani protozoa is responsible for human visceral leishmaniasis. During their life cycle, the parasites exist in 2 forms: as flagellated promastigote within the sandfly vector and as non flagellated amastigote in the macrophage phagolysosomal compartment of the mammalian host. A2 protein is an amastigote specific virulence factor that is required for Leishmania donovani parasite survival in a mammalian host.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab150344 は 3 報の論文で使用されています。
- Papadaki A et al. The Leishmania donovani LDBPK_220120.1 Gene Encodes for an Atypical Dual Specificity Lipid-Like Phosphatase Expressed in Promastigotes and Amastigotes; Substrate Specificity, Intracellular Localizations, and Putative Role(s). Front Cell Infect Microbiol 11:591868 (2021). PubMed: 33842381
- Giri S & Shaha C Leishmania donovani parasite requires Atg8 protein for infectivity and survival under stress. Cell Death Dis 10:808 (2019). PubMed: 31649242
- Domínguez-Bernal G et al. HisAK70: progress towards a vaccine against different forms of leishmaniosis. Parasit Vectors 8:629 (2015). PubMed: 26653170
