AB183028

Anti-Legumain 抗体 [EPR14718]

Name: Anti-Legumain 抗体 [EPR14718] (ab183028) | Abcam
Brand: Abcam Limited
SKU: AB183028
Price: 44000 JPY
Availability: InStock
Rating: 1 (1 reviews)

Anti-Legumain antibody [EPR14718]

RabMAb
Recombinant
詳細を見る

(1 Review)

(8 Publications)

Rabbit Recombinant Monoclonal Legumain antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications.

別名を表示する

PRSC1, LGMN, Legumain, Asparaginyl endopeptidase, AEP

1 Images

Lab

Western blot - Anti-Legumain antibody [EPR14718] (AB183028)

Actual band at : 36kDa, 46kDa, 56kDa

All lanes:

Western blot - Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 20 µg

Lane 2:

JAR (human placenta choriocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Predicted band size: 49 kDa

false

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14718

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Legumain also known as asparaginyl endopeptidase is an enzyme that plays a mechanical role as a protease. This enzyme has a molecular mass of approximately 56 kDa and it functions by catalyzing the hydrolysis of asparagine residues. Legumain is expressed in various tissues with higher levels observed in kidneys placenta and liver. Its proteolytic activity is essential for the breakdown of intracellular proteins within lysosomes and endosomal compartments.

Biological function summary

Legumain contributes to protein degradation and antigen processing. It does not operate alone as it is part of larger protein complexes which enhance its stability and function. Through its role in proteolysis legumain influences cellular processes like maturation of endolysosomal enzymes and processing of proenzymes. Additionally it assists in activating other proteases signifying its critical position in maintaining cellular proteostasis.

Pathways

Legumain participates in the regulation of immune responses and cellular homeostasis. It plays a significant role in the lysosomal pathway where it partners with cathepsins to assist in the degradation of proteins. Additionally legumain influences the MHC class II pathway by contributing to the cleavage of invariant chain aiding antigen presentation. Its interaction with cathepsins and the impact on antigen processing indicates its necessity in immune system functionality.

Legumain has been linked to disorders such as cancer and neurodegeneration. In cancer elevated legumain levels correlate with tumor invasiveness and metastasis especially in association with proteins like matrix metalloproteinases. Meanwhile in neurodegenerative conditions its dysregulation may contribute to abnormal protein accumulation and synaptic dysfunction. Understanding legumain's role and interactions can guide therapeutic strategies for these diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed : 23776206). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed : 23776206). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (PubMed : 9872320). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (By similarity). Required for normal lysosomal protein degradation in renal proximal tubules (By similarity). Required for normal degradation of internalized EGFR (By similarity). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity).

See full target information LGMN

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:2060-2075 PubMed40527988

2025

DMXL1 promotes recruitment of V1-ATPase to lysosomes upon TRPML1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Lee,Matthew J G Eldridge,Miguel A Gonzalez-Lozano,Thomas Bresnahan,Zachary Niday,Donato Del Camino,Tao Fu,Joao A Paulo,Magdalene M Moran,Sophie Helaine,J Wade Harper

Cell death & disease 16:93 PubMed39948060

2025

HIF2α drives ccRCC metastasis through transcriptional activation of methylation-controlled J protein and enhanced prolegumain secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Shen,Yu Su,Dekun Wang,Gang Li,Xuan Liu,Chuangxin Sun,Taoyu Hu,Haoxiang Pang,Xue Mi,Yuying Zhang,Shijing Yue,Zhujun Zhang,Xiaoyue Tan

ACS applied materials & interfaces 16:11159-11171 PubMed38385360

2024

Cell-Penetrating and Enzyme-Responsive Peptides for Targeted Cancer Therapy: Role of Arginine Residue Length on Cell Penetration and In Vivo Systemic Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Behnaz Ghaemi,Swati Tanwar,Aruna Singh,Dian R Arifin,Michael T McMahon,Ishan Barman,Jeff W M Bulte

Neural regeneration research 19:2081-2088 PubMed38227539

2024

Gut microbiota dysbiosis contributes to α-synuclein-related pathology associated with C/EBPβ/AEP signaling activation in a mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Fang,Sha Liu,Bilal Muhammad,Mingxuan Zheng,Xing Ge,Yan Xu,Shu Kan,Yang Zhang,Yinghua Yu,Kuiyang Zheng,Deqin Geng,Chun-Feng Liu

Cell death & disease 12:65 PubMed33431801

2021

Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan'ai Chen,Dekun Wang,Yangyang Yu,Tianyuan Zhao,Ningning Min,Yan Wu,Lichun Kang,Yong Zhao,Lingfang Du,Mianzhi Zhang,Junbo Gong,Zhujun Zhang,Yuying Zhang,Xue Mi,Shijing Yue,Xiaoyue Tan

Behavioural brain research 376:112219 PubMed31509774

2019

Legumain acts on neuroinflammatory to affect CUS-induced cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxing Lian,Kai Li,Jing Gao,Xiaoyue Tan,Zhuo Yang

Experimental neurology 306:1-9 PubMed29673933

2018

Lysophosphatidic acid induces neuronal cell death via activation of asparagine endopeptidase in cerebral ischemia-reperfusion injury.

Applications

Species

Rat

Chao Wang,Jie Zhang,Junchun Tang,Yi-Yi Li,YanXia Gu,Ying Yu,Jing Xiong,Xueqing Zhao,Zheng Zhang,Ting-Ting Li,Jutao Chen,Qi Wan,Zhaohui Zhang

Oncotarget 8:73793-73809 PubMed29088746

2017

Cystatin C deficiency suppresses tumor growth in a breast cancer model through decreased proliferation of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Janja Završnik,Miha Butinar,Mojca Trstenjak Prebanda,Aleksander Krajnc,Robert Vidmar,Marko Fonović,Anders Grubb,Vito Turk,Boris Turk,Olga Vasiljeva

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Legumain 抗体 [EPR14718]

Anti-Legumain antibody [EPR14718]

Western blot - Anti-Legumain antibody [EPR14718] (AB183028)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

代替製品

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise