Anti-LEFTY2 抗体 [7C5G1H6H10] (ab34593)
![Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (ab34593)](https://www.abcam.co.jp/ps/products/34/ab34593/Images/ab34593wb.bmp "Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (ab34593)")
Key features and details
- Mouse monoclonal [7C5G1H6H10] to LEFTY2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-LEFTY2 antibody [7C5G1H6H10]
LEFTY2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7C5G1H6H10] to LEFTY2 -
由来種
Mouse -
特異性
This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Fusion protein corresponding to Human Lefty. Recombinant 6X HIS tagged human Lefty.
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特記事項
Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. -
ポリ/モノ
モノクローナル -
クローン名
7C5G1H6H10 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34593の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 41 kDa).
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| 特記事項 |
|---|
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WB
1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 41 kDa). |
ターゲット情報
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機能
Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding. -
組織特異性
Mesenchymal cells of the endometrial stroma. -
関連疾患
Defects in LEFTY2 are the cause of left-right axis malformations (LRAM) [MIM:601877]. The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava. -
配列類似性
Belongs to the TGF-beta family. -
発生段階
Transiently expressed before and during menstrual bleeding. -
翻訳後修飾
The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7044 Human
- Entrez Gene: 320202 Mouse
- Entrez Gene: 289316 Rat
- Omim: 601877 Human
- SwissProt: O00292 Human
- SwissProt: P57785 Mouse
- Unigene: 520187 Human
- Unigene: 87078 Mouse
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別名
- EBAF antibody
- Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily) antibody
- Endometrial bleeding associated factor antibody
see all
画像
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Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (ab34593)All lanes : Anti-LEFTY2 antibody [7C5G1H6H10] (ab34593) at 1/2000 dilution
Lane 1 : 1µg 6X His tagged unrelated protein (shows that the antibody does not recognize the epitope tag).
Lane 2 : 1µg partially purified recombinant 6X his tagged human Lefty
Secondary
All lanes : IRDyeTM800 Goat anti-mouse IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab34593 は論文での使用が確認できていません。