Anti-Lefty 抗体 (ab22569)
Key features and details
- Rabbit polyclonal to Lefty
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Lefty antibody
Lefty 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Lefty -
由来種
Rabbit -
特異性
This antibody has been tested against recombinant protein only (human LeftyA and mouse Lefty1). I is expected to cross react with Human LeftyB as there is 100% homology of the immunogen with Human LeftyB. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
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ポジティブ･コントロール
- ICC/IF: mES cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22569の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 44,38,34 kDa (predicted molecular weight: 41 kDa).
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44,38,34 kDa (predicted molecular weight: 41 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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関連性
During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 10637 Human
- Entrez Gene: 13590 Mouse
- Omim: 603037 Human
- SwissProt: O75610 Human
- SwissProt: Q64280 Mouse
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別名
- AI450052 antibody
- Ebaf antibody
- Left right determination factor 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Lefty antibody (ab22569)
ab22569 staining Lefty in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab22569 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Western blot - Anti-Lefty antibody (ab22569)All lanes : Anti-Lefty antibody (ab22569) at 1 µg/ml
Lane 1 : Mouse Lefty1 Recombinant Protein
Lane 2 : Human LeftyA Recombinant Protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 34,38,44 kDa why is the actual band size different from the predicted?
The mouse recombinant Lefty protein consists of two bands of ~34 and 12 kDa. Of these, ab22569 recognises the 34 kDa band. The human recombinant Lefty protein consists of three bands of 44, 38 and 34 kDa. Of these, the first two are recognised by ab22569. The protein sizes differ from the expected molecular weight of Lefty due to proteolytic processing and glycosylation.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab22569 は 9 報の論文で使用されています。
- Lim J et al. Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally. Sci Rep 13:8671 (2023). PubMed: 37248279
- Zhang Q et al. A human Barrett's esophagus organoid system reveals epithelial-mesenchymal plasticity induced by acid and bile salts. Am J Physiol Gastrointest Liver Physiol 322:G598-G614 (2022). PubMed: 35380457
- Harpelunde Poulsen K et al. Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours. BMC Cancer 20:349 (2020). PubMed: 32326899
- Gambichler T et al. Expression of Lefty predicts Merkel cell carcinoma-specific death. J Eur Acad Dermatol Venereol 34:2016-2020 (2020). PubMed: 32022949
- Zhang P et al. Dissecting the Single-Cell Transcriptome Network Underlying Gastric Premalignant Lesions and Early Gastric Cancer. Cell Rep 27:1934-1947.e5 (2019). PubMed: 31067475
- Xu C et al. Lefty1 alleviates renal tubulointerstitial injury in mice with unilateral ureteral obstruction. Mol Med Rep 13:901-8 (2016). IHC-P ; Mouse . PubMed: 26647776
- Yoon SJ et al. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun 6:6546 (2015). WB, IP ; Mouse . PubMed: 25775035
- Sun G et al. Lefty inhibits glioma growth by suppressing Nodal-activated Smad and ERK1/2 pathways. J Neurol Sci 347:137-42 (2014). PubMed: 25304053
- Barroso-delJesus A et al. The Nodal inhibitor Lefty is negatively modulated by the microRNA miR-302 in human embryonic stem cells. FASEB J 25:1497-508 (2011). WB, IHC (PFA fixed) ; Human . PubMed: 21266536