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リコンビナントRabMAb

Anti-LEF1 抗体 [EPR24254-129] - BSA and Azide free (Capture) (ab300711)

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Sandwich ELISA - Anti-LEF1 antibody [EPR24254-129] - BSA and Azide free (Capture) (ab300711)
  • Anti-LEF1 [EPR24254-159] - BSA and Azide free (Capture) (AB300711)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24254-129] to LEF1 - BSA and Azide free (Capture)
  • Suitable for: Sandwich ELISA
  • Reacts with: Mouse, Human

製品の概要

  • 製品名

    Anti-LEF1 antibody [EPR24254-129] - BSA and Azide free (Capture)
    LEF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24254-129] to LEF1 - BSA and Azide free (Capture)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    Ab300711 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISA to quantify human LEF1. The recommended pair for sandwich ELISA is:
         Capture: ab300711, human LEF1 Capture Antibody (unconjugated)
         Detector: ab300712, human LEF1 Detector Antibody (unconjugated)
    The reference range value is 78 - 5000 pg/mL.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300711の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24254-159] to LEF1 - BSA and Azide free (Detector) (ab300712).
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24254-159] to LEF1 - BSA and Azide free (Detector) (ab300712).

ターゲット情報

  • 機能

    Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

  • 組織特異性

    Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

  • 配列類似性

    Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.

  • ドメイン

    Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.

  • 細胞内局在

    Nucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DKFZp586H0919 antibody
      • FLJ46390 antibody
      • LEF 1 antibody
      • LEF-1 antibody
      • Lef1 antibody
      • LEF1_HUMAN antibody
      • Lymphoid enhancer binding factor 1 antibody
      • Lymphoid enhancer-binding factor 1 antibody
      • T cell specific transcription factor 1 alpha antibody
      • T cell-specific transcription factor 1-alpha antibody
      • TCF 1 alpha antibody
      • TCF1 alpha antibody
      • TCF1-alpha antibody
      • TCF10 antibody
      • TCF1alpha antibody
      • TCF7L3 antibody
      • Transcription factor T cell specific 1 alpha antibody
      see all

    画像

    • Sandwich ELISA - Anti-LEF1 antibody [EPR24254-129] - BSA and Azide free (Capture) (ab300711)
      Sandwich ELISA - Anti-LEF1 antibody [EPR24254-129] - BSA and Azide free (Capture) (ab300711)
      Sandwich ELISA of ab300710 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
    • Anti-LEF1 [EPR24254-159] - BSA and Azide free (Capture) (AB300711)
      Anti-LEF1 [EPR24254-159] - BSA and Azide free (Capture) (AB300711)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300711 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300711 は論文での使用が確認できていません。

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