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AB167416

Anti-LDLC 抗体 [EPR10896]

Anti-LDLC antibody [EPR10896]

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(1 Publication)

Rabbit Recombinant Monoclonal LDLC antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

LDLC, COG2, Conserved oligomeric Golgi complex subunit 2, COG complex subunit 2, Component of oligomeric Golgi complex 2, Low density lipoprotein receptor defect C-complementing protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling LDLC with ab167416 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-LDLC antibody [EPR10896] (AB167416)
  • WB

Unknown

Western blot - Anti-LDLC antibody [EPR10896] (AB167416)

All lanes:

Western blot - Anti-LDLC antibody [EPR10896] (ab167416) at 1/1000 dilution

Lane 1:

Fetal lung tissue lysate at 10 µg

Lane 2:

Fetal brain tissue lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 83 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LDLC with ab167416 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10896

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LDLC also known as Low-Density Lipoprotein Cholesterol is a significant component of the plasma lipid transport system. It plays a mechanical role in transporting cholesterol to peripheral tissues. The molecular mass of LDLC varies depending on the size of the lipoprotein particles typically ranging from 2000 to 3000 kDa. LDLC prominently circulates in the blood and is widely expressed throughout the body particularly in liver and intestines where it is synthesized and secreted.
Biological function summary

LDLC serves as the primary cholesterol carrier and is essential for cellular membrane synthesis and maintenance. It forms a complex with apolipoproteins such as ApoB-100 which facilitate interaction with LDL receptors on cell surfaces. This interaction enables the uptake of cholesterol for use in intracellular processes. LDL particles derive from very low-density lipoproteins (VLDL) following triglyceride depletion emphasizing their role in lipid metabolism.

Pathways

The cholesterol transport and lipid metabolism pathways involve LDLC as a core component. One important pathway is the mevalonate pathway where it links to biosynthesis of cholesterol steroids and isoprenoids. The protein SREBP (Sterol Regulatory Element Binding Protein) regulates the expression of LDL receptors impacting cholesterol uptake efficiency. Another relevant pathway is the reverse cholesterol transport pathway involving ApoA-I where excess cholesterol from tissues returns to the liver for excretion.

LDLC is strongly associated with cardiovascular diseases especially atherosclerosis. Elevated levels of LDLC contribute to plaque formation in arterial walls leading to heart diseases. Familial hypercholesterolemia a genetic disorder also links to increased LDLC levels due to defective LDL receptors caused by mutations in the LDLR gene. Apolipoprotein E (ApoE) polymorphisms further affect the clearance rate of LDLC influencing susceptibility to these conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Required for normal Golgi morphology and function.
See full target information COG2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 39: PubMed30988160

2019

Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.

Applications

Unspecified application

Species

Unspecified reactive species

David McClellan,Mattie J Casey,Diana Bareyan,Helena Lucente,Christopher Ours,Matthew Velinder,Jason Singer,Mehraju Din Lone,Wenxiang Sun,Yunuen Coria,Clinton C Mason,Michael E Engel
View all publications
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組合せ製品

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Multiplex Kits

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Multiplex Kits

AB193659

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 96 Targets)

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Abcam product promise

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