Anti-LDL Receptor 抗体 (ab30532)
Key features and details
- Rabbit polyclonal to LDL Receptor
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LDL Receptor antibody
LDL Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LDL Receptor -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide:
SVADTKGVKRRTL
, corresponding to amino acids 499-511 of Mouse LDL Receptor -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 40% Glycerol, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30532の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/400.
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| ICC/IF | (1) |
1/100 - 1/400.
|
| WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 95 kDa.
|
| 特記事項 |
|---|
|
IHC-P
1/400. |
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ICC/IF
1/100 - 1/400. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 95 kDa. |
ターゲット情報
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機能
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. -
関連疾患
Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). -
配列類似性
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
翻訳後修飾
N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation. -
細胞内局在
Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments. - Information by UniProt
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参照データベース
- Entrez Gene: 3949 Human
- Entrez Gene: 16835 Mouse
- Entrez Gene: 300438 Rat
- Omim: 606945 Human
- SwissProt: P01130 Human
- SwissProt: P35951 Mouse
- SwissProt: P35952 Rat
- Unigene: 213289 Human
see all -
別名
- FH antibody
- FHC antibody
- LDL R antibody
see all
画像
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Immunocytochemistry - Anti-LDL Receptor antibody (ab30532)This image is courtesy of an anonymous AbreviewImmunocytochemistry analysis of acetone-fixed human Liver cells, Hep2G staining with ab30532 at 1/200 dilution. Secondary antibody was FITC rabbit IgG at 1/400 dilution. Samples were incubated with the primary antibody with Serum/PBS for 1 hours at 21°C. Blocking was done using 5% serum for 15 minutes at 21°C.
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Western blot - Anti-LDL Receptor antibody (ab30532)All lanes : Anti-LDL Receptor antibody (ab30532) at 1/200 dilution
Lane 1 : RAW 264.7 Cells at 25 µg
Lane 2 : RAW 264.7 Cells at 50 µg
Lane 3 : Rat Liver Supernatant at 50 µg
Secondary
All lanes : Anti-Rabbit-IgG-HRP at 1/7000 dilution
Developed using the ECL technique.
Predicted band size: 95 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody (ab30532)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded (FFPE) mouse lung tissue after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with LDL receptor polyclonal antibody, ab30532, at a 1:100 dilution, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen (DAB).
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Western blot - Anti-LDL Receptor antibody (ab30532)All lanes : Anti-LDL Receptor antibody (ab30532)
Lane 1 : HeLa Cell Lysate
Lane 2 : HepG2 Cell Lysate
Lane 3 : Huh7 Cell Lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Mouse monoclonal [6F12] to Cytomegalovirus (ab6500) (Anti-Rabbit IgG (HRP) conjugate)
Predicted band size: 95 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody (ab30532)ab30532 staining LDL receptor in human lung parenchyma.
Left panel: with primary antibody at 1/400. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-LDL Receptor antibody (ab30532)ICC/IF image of ab30532 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30532, 1/400) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (88)
ab30532 は 88 報の論文で使用されています。
- Li S et al. Resveratrol Regulates Glucose and Lipid Metabolism in Diabetic Rats by Inhibition of PDK1/AKT Phosphorylation and HIF-1α Expression. Diabetes Metab Syndr Obes 16:1063-1074 (2023). PubMed: 37090841
- Liu K et al. Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their function. Nat Commun 14:4007 (2023). PubMed: 37414857
- Lopez-Lopez A et al. Interactions between Angiotensin Type-1 Antagonists, Statins, and ROCK Inhibitors in a Rat Model of L-DOPA-Induced Dyskinesia. Antioxidants (Basel) 12:N/A (2023). PubMed: 37507992
- Hanse M et al. Garcinia cambogia Extract Increased Hepatic Levels of Lipolysis-Stimulated Lipoprotein Receptor and Lipids in Mice on Normal Diet. Int J Mol Sci 24:N/A (2023). PubMed: 38003494
- Barcena ML et al. The impact of the PCSK-9/VLDL-Receptor axis on inflammatory cell polarization. Cytokine 161:156077 (2023). PubMed: 36356495