JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB244816

Anti-LC3A 抗体 [EPR20313-267] - BSA and Azide free (Capture)

Anti-LC3A antibody [EPR20313-267] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Microtubule-associated proteins 1A/1B light chain 3A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

別名を表示する

Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A

1 Images
Sandwich ELISA - Anti-LC3A antibody [EPR20313-267] - BSA and Azide free (Capture) (AB244816)
  • sELISA

Unknown

Sandwich ELISA - Anti-LC3A antibody [EPR20313-267] - BSA and Azide free (Capture) (AB244816)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab239432).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20313-267

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

ab244816 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LC3A. The recommended pair for sandwich ELISA is:
Capture: ab244816, Human LC3A Capture Antibody (unconjugated)
Detector: ab245089, Human LC3A Detector Antibody (unconjugated)
The reference range value is 39.06 - 2500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein LC3A also known as Microtubule-associated proteins 1A/1B light chain 3A plays an important role in the autophagy process. It exists in two forms LC3-I and LC3-II with the latter being lipidated and integrated into autophagosomal membranes. LC3A has a molecular mass of approximately 17 kDa. Researchers observe its expression in various tissues but most highly in muscle liver and brain. It is often studied along with LC3B due to their similar roles in autophagy.
Biological function summary

LC3A participates in the formation of autophagosomes which are double-membraned vesicles that capture cellular waste for degradation. LC3A's conversion from LC3-I to LC3-II on the autophagosomal membrane signals the initiation of autophagy. It associates with proteins in the Atg family forming complexes that regulate the maturation of autophagosomes. The LC3 family including LC3A and LC3B acts as important components in the cell's ability to maintain homeostasis through autophagic activity.

Pathways

LC3A plays a vital part in the autophagy pathway and is also linked to the mTOR signaling pathway. Autophagy is a process that helps with the recycling of cellular components and mTOR signaling inhibits autophagy when nutrients are abundant. LC3A's interactions with other proteins such as p62 and ULK1 are essential in modulating these processes and ensuring cell survival under stress conditions. LC3A and LC3B's roles are integral in coordinating the cellular response to nutrient status and stress highlighting their importance in cellular regulation.

LC3A has significant connections to cancer and neurodegenerative diseases. Altered autophagic activity where LC3A takes part appears in various forms of cancer indicating its role in tumor progression and apoptosis regulation. Additionally abnormal LC3A function has implications in disorders such as Alzheimer's disease where autophagic dysfunction leads to neurodegeneration. In these contexts LC3A's relationship with proteins like p62 which accumulate in neurodegenerative diseases provides insight into pathological mechanisms and potential therapeutic targets.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20713600, PubMed : 24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538).
See full target information MAP1LC3A
style
left-column
style
left-column
style
right-column

組合せ製品

Assay Kits

AB239432

Human LC3A ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human LC3A ELISA Kit (AB239432)

  • 0
  • 0
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
Multiplex Kits

AB228525

Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

  • 5
  • 1
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
Primary Antibodies

AB133528

Anti-ATG7 antibody [EPR6251]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ATG7 antibody [EPR6251] (AB133528)

  • 4
  • 16

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com