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AB254559

Anti-LBP 抗体 [EPR22559-261]

Anti-LBP antibody [EPR22559-261]

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(2 Publications)

Rabbit Recombinant Monoclonal LBP antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications.

別名を表示する

Lipopolysaccharide-binding protein, LBP, Lbp

4 Images
Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (AB254559)
  • IP

Unknown

Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (AB254559)

LBP was immunoprecipitated from 0.35 mg mouse ovary lysate with ab254559 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab254559 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1 : Mouse ovary lysate 10μg (Input).
Lane 2 : ab254559 IP in mouse ovary lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254559 in mouse ovary lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 20 seconds.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (ab254559)

Predicted band size: 53 kDa

Observed band size: 60 kDa

false

Western blot - Anti-LBP antibody [EPR22559-261] (AB254559)
  • WB

Unknown

Western blot - Anti-LBP antibody [EPR22559-261] (AB254559)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 59 seconds

The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID : 7694297)

Negative control : SP2/0-Ag14 (PMID : 2456339).

All lanes:

Western blot - Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution

Lane 1:

SP2/0-Ag14 (mouse myeloma cell line), whole cell lysate at 20 µg

Lane 2:

Mouse ovary tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 60 kDa

false

Western blot - Anti-LBP antibody [EPR22559-261] (AB254559)
  • WB

Unknown

Western blot - Anti-LBP antibody [EPR22559-261] (AB254559)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 59 seconds

The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID : 7694297).

All lanes:

Western blot - Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse plasma at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 60 kDa

false

Western blot - Anti-LBP antibody [EPR22559-261] (AB254559)
  • WB

Unknown

Western blot - Anti-LBP antibody [EPR22559-261] (AB254559)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution

All lanes:

His-tagged full-length recombinant mouse LBP protein 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 60 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-LBP antibody [EPR22559-261] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22559-261

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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下記製品にもご興味をお持ちいただけるかもしれません:

AB269542

Mouse LBP ELISA Kit (LPS Binding Protein)

5

1 Reviews

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お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Lipopolysaccharide-binding protein (LBP) also known as LPS-binding protein is an acute-phase protein that plays a critical role in the immune response to Gram-negative bacterial infections. This protein weighing approximately 60kDa is primarily expressed in the liver and found in the serum. LBP binds to lipopolysaccharides (LPS) which are components of the outer membrane of Gram-negative bacteria facilitating the transfer of LPS to CD14 receptors on immune cells. This transfer enhances the recognition of bacterial endotoxins triggering an immune response.
Biological function summary

LBP serves a significant role in enhancing the sensitivity of the immune system to bacterial endotoxins. It does not form a permanent part of a larger complex but works closely with other molecules like CD14 and MD-2 an accessory protein of the TLR4 receptor to mediate the innate immune response. By efficiently binding LPS LBP aids in transporting these molecules to cell surface receptors accelerating the detection and subsequent response to infections.

Pathways

LBP is heavily involved in the TLR4 signaling pathway which is important for the detection and response to Gram-negative bacterial infections. Through this pathway LBP and associated proteins such as CD14 and MD-2 help activate TLR4 triggering downstream NF-κB signaling. This activation leads to the production of pro-inflammatory cytokines and an upsurge in antimicrobial activities fortifying the body's defenses against bacterial invasion.

Enhanced LBP levels have been associated with sepsis a severe systemic response to bacterial infections which can often lead to septic shock. Given its pivotal role in recognizing bacterial endotoxins LBP is important in identifying infections making it a potential prospect for therapeutic targeting. Additionally its interaction with CD14 means that alterations in LBP function or expression levels might contribute to immune dysregulation seen in inflammatory diseases such as Crohn's disease where immune responses to gut microbiota play a central role.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed : 9144073). Acts as an affinity enhancer for CD14, facilitating its association with LPS (By similarity). Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed : 24380872).
See full target information Lbp
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 52: PubMed37830152

2023

Intestinal changes in permeability, tight junction and mucin synthesis in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jing He,Yuanjie Liu,Junhua Li,Yueyang Zhao,Hanxiao Jiang,Shifang Luo,Guiqiong He

Cell cycle (Georgetown, Tex.) 22:967-985 PubMed36710409

2023

Deficiency of lipopolysaccharide binding protein facilitates adipose browning, glucose uptake and oxygen consumption in mouse embryonic fibroblasts via activating PI3K/Akt/mTOR pathway and inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyao Yin,Zhiye Xu,Xinxin Zhang,Jiahua Wu,Weina Lu
View all publications
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保証に関する詳細については利用規約をご確認ください。
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