Anti-LAT (phospho Y200) 抗体 [EP983(2)Y] (ab68139)
![Western blot - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139)](https://www.abcam.co.jp/ps/products/68/ab68139/Images/ab68139-511402-anti-lat-phospho-y200-antibody-ep983-2-y-western-blot-human-jurkat.jpg "Western blot - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP983(2)Y] to LAT (phospho Y200)
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LAT (phospho Y200) antibody [EP983(2)Y]
LAT 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP983(2)Y] to LAT (phospho Y200) -
由来種
Rabbit -
特異性
The antibody only detects LAT phosphorylated on Tyrosine 200 of long isoform and Tyrosine 171 of short isoform.
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アプリケーション
適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Jurkat cells treated with 1mM pervanadate. ICC/IF: Jurkat cells. Flow Cyt (Intra): Jurkat cells.
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特記事項
This product has switched from a hybridoma to recombinant production method on 31st May 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP983(2)Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68139の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 35 kDa.
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| ICC/IF |
1/250.
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| Flow Cyt (Intra) |
1/2000.
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| 特記事項 |
|---|
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WB
1/10000. Predicted molecular weight: 35 kDa. |
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ICC/IF
1/250. |
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Flow Cyt (Intra)
1/2000. |
ターゲット情報
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機能
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. -
組織特異性
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). -
翻訳後修飾
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. -
細胞内局在
Cell membrane. Present in lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 27040 Human
- Omim: 602354 Human
- SwissProt: O43561 Human
- Unigene: 632179 Human
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別名
- 36 kDa phospho tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adapter protein antibody
- 36 kDa phospho-tyrosine adaptor protein antibody
see all
画像
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Western blot - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139)All lanes : Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139) at 1/10000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with 1mM pervanadate for 30 min whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 7 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139)](https://www.abcam.co.jp/ps/products/68/ab68139/Images/ab68139-511401-anti-lat-phospho-y200-antibody-ep983-2-y-immunocytochemistry-human-jurkat.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat cells labelling LAT with ab68139 at 1/250 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing no staining in Jurkat cells and positive staining in Jurkat cells treated with pervanadate 1mM for 30 mins. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139)](https://www.abcam.co.jp/ps/products/68/ab68139/Images/ab68139-511400-anti-lat-phospho-y200-antibody-ep983-2-y-flow-cytometry-human-jurkat.jpg)
Flow Cytometry (Intracellular) - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139)Flow cytometric analysis of 4% paraformaldehyde fixed bLA permeabilized Jurkat cells labelling LAT with ab68139 at 1:2000 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab68139 は 8 報の論文で使用されています。
- Jooss NJ et al. Anti-GPVI nanobody blocks collagen- and atherosclerotic plaque-induced GPVI clustering, signaling, and thrombus formation. J Thromb Haemost 20:2617-2631 (2022). PubMed: 35894121
- Alatawi KA et al. 1,8-Cineole Affects Agonists-Induced Platelet Activation, Thrombus Formation and Haemostasis. Cells 10:N/A (2021). PubMed: 34685597
- Dunster JL et al. Interspecies differences in protein expression do not impact the spatiotemporal regulation of glycoprotein VI mediated activation. J Thromb Haemost 18:485-496 (2020). PubMed: 31680418
- Donner L et al. The collagen receptor glycoprotein VI promotes platelet-mediated aggregation of ß-amyloid. Sci Signal 13:N/A (2020). PubMed: 32753479
- Flora GD et al. Non-genomic effects of the Pregnane X Receptor negatively regulate platelet functions, thrombosis and haemostasis. Sci Rep 9:17210 (2019). PubMed: 31748641
- Roweth HG et al. Citalopram inhibits platelet function independently of SERT-mediated 5-HT transport. Sci Rep 8:3494 (2018). PubMed: 29472624
- Haining EJ et al. Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation. Platelets 28:629-642 (2017). PubMed: 28032533
- Huang SC et al. Lipid raft assembly and Lck recruitment in TRAIL costimulation mediates NF-?B activation and T cell proliferation. J Immunol 186:931-9 (2011). PubMed: 21160038