Anti-LASS6/CERS6 抗体 (ab115539)
Key features and details
- Rabbit polyclonal to LASS6/CERS6
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-LASS6/CERS6 antibody -
製品の詳細
Rabbit polyclonal to LASS6/CERS6 -
由来種
Rabbit -
特異性
ab115539 is predicted not to cross-react with LASS5. -
アプリケーション
適用あり: ELISA, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115539の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be involved in sphingolipid synthesis or its regulation. -
配列類似性
Contains 1 homeobox DNA-binding domain.
Contains 1 TLC (TRAM/LAG1/CLN8) domain. -
翻訳後修飾
Glycosylation on Asn-18 is not necessary for function. -
細胞内局在
Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 253782 Human
- Entrez Gene: 241447 Mouse
- Entrez Gene: 366065 Rat
- SwissProt: Q6ZMG9 Human
- SwissProt: Q8C172 Mouse
- Unigene: 506829 Human
- Unigene: 724377 Human
- Unigene: 222222 Mouse
see all -
別名
- Ceramide synthase 6 antibody
- CerS 6 antibody
- CERS5 antibody
see all
画像
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Western blot - Anti-LASS6/CERS6 antibody (ab115539)Lane 1 : Anti-LASS6/CERS6 antibody (ab115539) at 1 µg/ml
Lane 2 : Anti-LASS6/CERS6 antibody (ab115539) at 2 µg/ml
All lanes : Rat brain tissue lysate
Predicted band size: 45 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS6/CERS6 antibody (ab115539)ab115539, at 5µg/ml, staining LASS6/CERS6 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS6/CERS6 antibody (ab115539)ab115539, at 5µg/ml, staining LASS6/CERS6 in Formalin-fixed, Paraffin-embedded Human Small intestine tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS6/CERS6 antibody (ab115539)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling LASS6/CERS6 with ab115539 at 5µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab115539 は 2 報の論文で使用されています。
- Shi Y et al. Ceramide synthase 6 predicts poor prognosis and activates the AKT/mTOR/4EBP1 pathway in high-grade serous ovarian cancer. Am J Transl Res 12:5924-5939 (2020). PubMed: 33042469
- Ghandour B et al. Restoration of ceramide de novo synthesis by the synthetic retinoid ST1926 as it induces adult T-cell leukemia cell death. Biosci Rep 40:N/A (2020). PubMed: 33048123