Anti-Lass5 抗体 (ab115314)
Key features and details
- Rabbit polyclonal to Lass5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Lass5 antibody
Lass5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Lass5 -
由来種
Rabbit -
特異性
Multiple isoforms of Lass5 are known to exist. This antibody may cross-react with the highly homologous Lass6. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide, 17 amino acids, from the C terminal region of Human Lass5.
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ポジティブ･コントロール
- Human small intestine tissue; SK-N-SH cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115314の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl- (C16) ceramide) in a fumonisin B1-independent manner. -
配列類似性
Contains 1 homeobox DNA-binding domain.
Contains 1 TLC (TRAM/LAG1/CLN8) domain. -
細胞内局在
Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 91012 Human
- Entrez Gene: 71949 Mouse
- Entrez Gene: 366984 Rat
- SwissProt: Q8N5B7 Human
- SwissProt: Q9D6K9 Mouse
- SwissProt: B2RYI9 Rat
- Unigene: 270525 Human
- Unigene: 390821 Mouse
see all -
別名
- CerS5 antibody
- FLJ25304 antibody
- LAG1 homolog ceramide synthase 5 antibody
see all
画像
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Western blot - Anti-Lass5 antibody (ab115314)All lanes : Anti-Lass5 antibody (ab115314) at 1 µg/ml
Lane 1 : SK-N-SH cell lysate
Lane 2 : SK-N-SH cell lysate with blocking peptide
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lass5 antibody (ab115314)ab115314 at 5ug/ml staining Lass5 in Human small intestine tissue by immunohistochemistry (FFPE). After primary incubation slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115314 は論文での使用が確認できていません。