Anti-LASP1 抗体 (ab117806)
Key features and details
- Rabbit polyclonal to LASP1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LASP1 antibody
LASP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LASP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide, corresponding to a region within amino acids 100-150 of Human LASP1 (NP_006139.1).
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ポジティブ･コントロール
- Whole cell lysate from HeLa, 293T and Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab117806 was affinity purified using an epitope specific to LASP1 immobilized on a solid support . -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117806の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types. -
配列類似性
Contains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain. -
細胞内局在
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 3927 Human
- Entrez Gene: 100173670 Orangutan
- Entrez Gene: 694950 Rhesus monkey
- Omim: 602920 Human
- SwissProt: Q14847 Human
- SwissProt: Q5R5W0 Orangutan
- Unigene: 728766 Human
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別名
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASP1 antibody (ab117806)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling LASP1 with ab117806 at 1 µg/mL concentration. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-LASP1 antibody (ab117806)All lanes : Anti-LASP1 antibody (ab117806) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-LASP1 antibody (ab117806)ab117806 at 1 µg/ml staining LASP1 by WB following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117806 at 6 µg/mg lysate.
Lane 2; IP using an antibody to a downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab117806 は 4 報の論文で使用されています。
- Niimi A et al. LASP1, CERS6, and Actin Form a Ternary Complex That Promotes Cancer Cell Migration. Cancers (Basel) 15:N/A (2023). PubMed: 37345118
- Hao Q & Zhang Z hsa_circRNA_000166 Facilitated Cell Growth and Limited Apoptosis through Targeting miR-326/LASP1 Axis in Colorectal Cancer. Gastroenterol Res Pract 2020:8834359 (2020). PubMed: 33376485
- Li W et al. Long non-coding RNA LINC00672 contributes to p53 protein-mediated gene suppression and promotes endometrial cancer chemosensitivity. J Biol Chem 292:5801-5813 (2017). PubMed: 28232485
- Hu JJ et al. Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells. Proteome Sci 12:13 (2014). WB ; Dog . PubMed: 24580839