AB117806

Anti-LASP1 抗体

Name: Anti-LASP1 抗体 (ab117806) | Abcam
Brand: Abcam Limited
SKU: AB117806
Price: 76000 JPY
Availability: InStock

Anti-LASP1 antibody

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal LASP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human LASP1 aa 100-150.

別名を表示する

MLN50, LASP1, LIM and SH3 domain protein 1, LASP-1, Metastatic lymph node gene 50 protein, MLN 50

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASP1 antibody (AB117806)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASP1 antibody (AB117806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling LASP1 with ab117806 at 1 μg/mL concentration. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-LASP1 antibody (AB117806)

ab117806 at 1 μg/ml staining LASP1 by WB following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117806 at 6 μg/mg lysate.
Lane 2; IP using an antibody to a downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.

All lanes:

Immunoprecipitation - Anti-LASP1 antibody (ab117806)

Predicted band size: 30 kDa

false

Unknown

Western blot - Anti-LASP1 antibody (AB117806)

All lanes:

Western blot - Anti-LASP1 antibody (ab117806) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from 293T cells at 50 µg

Lane 4:

Whole cell lysate from Jurkat cells at 50 µg

Predicted band size: 30 kDa

true

Exposure time: 30s

CiteAb

Western blot - Anti-LASP1 antibody (AB117806)

LASP1 western blot using anti-LASP1 antibody ab117806. Publication image and figure legend from Hu, J. J., Liu, Y. W., et al., 2014, Proteome Sci, PubMed 24580839.

ab117806 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab117806 please see the product overview.

Confirmation of RNA and protein expression levels by real-time quantitative PCR and western blot analysis (n = 3, per treatment type). (A) The expression of LASP1 and ferritin light and heavy chain mRNAs relative to that of GAPDH, the internal control, as detected by real-time PCR. (B) Expression of LASP1 and ferritin light and heavy chains, as assessed by western blot analysis. The signal corresponding to the protein bands was quantified using densitometric scanning and the relative protein abundance was determined after normalization with the levels of β-actin protein. Data are presented as mean ± SD and compared using the t-test for independent samples. *p <0.05, indicating significant differences between the two samples compared.

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB, IP

applications

免疫原

Synthetic Peptide within Human LASP1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q14847

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab117806 was affinity purified using an epitope specific to LASP1 immobilized on a solid support .

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The LASP1 protein also known as LIM and SH3 protein 1 is a 261 amino acid protein with an approximate mass of 29 kDa. It acts as an actin-binding protein involved in the dynamics of the cytoskeleton. LASP1 is expressed in various tissues with higher expression seen in the kidneys lungs and breast tissues. Its structure includes an LIM domain at the N-terminal and an SH3 domain at the C-terminal essential for interactions with other proteins and cytoskeletal components.

Biological function summary

LASP1 plays a significant role in cell migration and adhesion processes. It associates with cytoskeletal proteins such as F-actin helping in the stabilization and organization of the cytoskeleton. LASP1 forms part of complexes with zyxin and vasodilator-stimulated phosphoprotein (VASP) at focal adhesions contributing to cellular motility and signaling. It influences processes like cytokinesis and wound healing which rely on precise cytoskeletal rearrangements.

Pathways

Researchers have identified LASP1 as being involved in the ERK/MAPK and PI3K/AKT signaling pathways. It links to these pathways through interactions promoting cell proliferation and survival. This protein associates with other pathway-related proteins like Grb2 and Src which play roles in transmitting signals from cell surface receptors to the nucleus impacting gene expression and cell cycle progression.

LASP1 overexpression often associates with various cancers particularly breast cancer and colorectal cancer. In these contexts LASP1 contributes to tumor progression and metastasis. Its interaction with proteins such as EGFR and HER2 has widespread implications in oncogenic signaling. Understanding LASP1's role in disease can enhance diagnostic and therapeutic strategies targeting these malignancies.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).

See full target information LASP1

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37345118

2023

LASP1, CERS6, and Actin Form a Ternary Complex That Promotes Cancer Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Niimi,Siripan Limsirichaikul,Keiko Kano,Yasuyoshi Mizutani,Toshiyuki Takeuchi,Patinya Sawangsri,Dat Quoc Tran,Yoshiyuki Kawamoto,Motoshi Suzuki

Open life sciences 16:523-536 PubMed34124372

2021

Identification of circRNA circ-CSPP1 as a potent driver of colorectal cancer by directly targeting the miR-431/LASP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Minghao Li,Jianbin Zhuang,Di Kang,Yuzhuo Chen,Weiliang Song

Gastroenterology research and practice 2020:8834359 PubMed33376485

2020

hsa_circRNA_000166 Facilitated Cell Growth and Limited Apoptosis through Targeting miR-326/LASP1 Axis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Hao,Zhongtao Zhang

The Journal of biological chemistry 292:5801-5813 PubMed28232485

2017

Long non-coding RNA LINC00672 contributes to p53 protein-mediated gene suppression and promotes endometrial cancer chemosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Hua Li,Liyuan Zhang,Min Hu,Fang Li,Jieqiong Deng,Mingxing An,Siqi Wu,Rui Ma,Jiachun Lu,Yifeng Zhou

Proteome science 12:13 PubMed24580839

2014

Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells.

Applications

Species

Dog

Ji-Jie Hu,Ya-Wei Liu,Min-Yi He,Dan Jin,Hui Zhao,Bin Yu

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LASP1 抗体

Anti-LASP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASP1 antibody (AB117806)

Immunoprecipitation - Anti-LASP1 antibody (AB117806)

Western blot - Anti-LASP1 antibody (AB117806)

Western blot - Anti-LASP1 antibody (AB117806)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise