Anti-LARP1 抗体 (ab86359)
Key features and details
- Rabbit polyclonal to LARP1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LARP1 antibody
LARP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LARP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide corresponding to Human LARP1 aa 250-350.
Database link: Q6PKG0 -
ポジティブ･コントロール
- WB: HeLa, 293T and Jurkat whole cell lysate, IP: HeLa whole cell lysate. IHC: Human colon carcinoma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86359の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
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IP
Use at an assay dependent concentration. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
LARP1 belongs to the LARP family and contains 1 HTH La-type RNA-binding domain. -
参照データベース
- Entrez Gene: 23367 Human
- Omim: 612059 Human
- SwissProt: Q6PKG0 Human
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別名
- 1810024J12Rik antibody
- 3110040D16Rik antibody
- KIAA0731 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LARP1 antibody (ab86359)
Ab86359 staining LARP1 in human colon carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde- fixed, paraffin embedded tissue sections). Samples were incubated with primary antibody at 1:200 dilution (1μg/ml).
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Western blot - Anti-LARP1 antibody (ab86359)All lanes : Anti-LARP1 antibody (ab86359) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 124 kDaDetection: Chemiluminescence with an exposure time of 3 seconds
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Immunoprecipitation - Anti-LARP1 antibody (ab86359)Detection of human LARP1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: Rabbit polyclonal to LARP1 (ab86359) at 3 µg/mg lysate
Lane 2: Rabbit polyclonal to LARP1 (ab245635) at 3 µg/mg lysate
Lane 3: Control IgG
Detection: Chemiluminescence with an exposure time of 3 seconds. ab70386 was used at 0.4 ug/mL for Western blot.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab86359 は 15 報の論文で使用されています。
- Caruso M et al. The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation. Stem Cell Reports 18:254-268 (2023). PubMed: 36563686
- Li P et al. Circular RNA coiled-coil domain containing 66 regulates malignant development of papillary thyroid carcinoma by upregulating La ribonucleoprotein 1 via the sponge effect on miR-129-5p. Bioengineered 13:7181-7196 (2022). PubMed: 35264065
- Xiao Y et al. Circ_0047921 acts as the sponge of miR-1287-5p to stimulate lung cancer progression by regulating proliferation, migration, invasion, and glycolysis of lung cancer cells. World J Surg Oncol 20:108 (2022). PubMed: 35365169
- Farooq Z et al. The amino acid sensor GCN2 suppresses terminal oligopyrimidine (TOP) mRNA translation via La-related protein 1 (LARP1). J Biol Chem 298:102277 (2022). PubMed: 35863436
- Wang CL et al. Long non-coding RNA ARHGAP5-AS1 inhibits migration of breast cancer cell via stabilizing SMAD7 protein. Breast Cancer Res Treat 189:607-619 (2021). PubMed: 34370213