Anti-LAP2 抗体 (ab185718)
Key features and details
- Rabbit polyclonal to LAP2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-LAP2 antibody
LAP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LAP2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human LAP2 aa 1 to the C-terminus.
Database link: P42167 -
ポジティブ･コントロール
- Extracts from 22RV1, K562, HepG2, 293T, SH-SY5Y and H460 cell lines.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185718の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/500 - 1/2000. Predicted molecular weight: 51 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/500 - 1/2000. Predicted molecular weight: 51 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 7112 Human
- Entrez Gene: 21917 Mouse
- Omim: 188380 Human
- SwissProt: P42166 Human
- SwissProt: P42167 Human
- SwissProt: Q61029 Mouse
- SwissProt: Q61033 Mouse
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別名
- Lamina associated polypeptide 2 antibody
- LAP 2 antibody
- LEM domain containing 4 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling LAP2 with ab185718 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification: 200x. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification: 400x. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification: 400x. -
Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (ab185718)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185718. Blue DAPI for nuclear staining. -
Western blot - Anti-LAP2 antibody (ab185718)All lanes : Anti-LAP2 antibody (ab185718) at 1/500 dilution
Lane 1 : Extract from 22RV1 cell line.
Lane 2 : Extract from K562 cell line.
Lane 3 : Extract from HepG2 cell line.
Lane 4 : Extract from 293T cell line.
Lane 5 : Extract from SH-SY5Y cell line.
Lane 6 : Extract from H460 cell line.
Predicted band size: 51 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab185718 は 3 報の論文で使用されています。
- Chen Y et al. The SUN1-SPDYA interaction plays an essential role in meiosis prophase I. Nat Commun 12:3176 (2021). PubMed: 34039995
- Li Z et al. TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO. Cancer Cell Int 20:294 (2020). PubMed: 32669970
- Mirza AN et al. LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription. Cell 176:198-212.e15 (2019). PubMed: 30503211