Anti-LAP2 alpha 抗体 (ab5162)
Key features and details
- Rabbit polyclonal to LAP2 alpha
- Suitable for: IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-LAP2 alpha antibody
LAP2 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LAP2 alpha -
由来種
Rabbit -
アプリケーション
適用あり: IP, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment (His-tag) corresponding to Human LAP2 alpha aa 188-693.
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特記事項
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: PBS, Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
一次抗体 備考
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5162の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
1/2500. Detects a band of approximately 83 kDa.
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特記事項 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
1/2500. Detects a band of approximately 83 kDa. |
ターゲット情報
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関連性
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 7112 Human
- Entrez Gene: 21917 Mouse
- Entrez Gene: 25359 Rat
- Omim: 188380 Human
- SwissProt: P42166 Human
- SwissProt: Q61033 Mouse
- SwissProt: Q62733 Rat
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別名
- Isoforms beta/gamma antibody
- Lamina associated polypeptide 2 antibody
- LAP2 antibody
see all
画像
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ab5162 at a dilution of 1/250 staining LAP 2 alpha in HeLa cells by immunofluorescence.
A = ab5162, 1/250
B = DNA
C = A and B merged
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ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL). ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab5162 は 25 報の論文で使用されています。
- Vidak S et al. Activation of endoplasmic reticulum stress in premature aging via the inner nuclear membrane protein SUN2. Cell Rep 42:112534 (2023). PubMed: 37210724
- Huo Q et al. SIRT7 Downregulation Promotes Breast Cancer Metastasis Via LAP2α-Induced Chromosomal Instability. Int J Biol Sci 19:1528-1542 (2023). PubMed: 37056924
- Harhouri K et al. MG132 Induces Progerin Clearance and Improves Disease Phenotypes in HGPS-like Patients' Cells. Cells 11:N/A (2022). PubMed: 35203262
- Maurizi E et al. Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity. Int J Mol Sci 23:N/A (2022). PubMed: 35628669
- Ding B et al. Disease Modeling with Human Neurons Reveals LMNB1 Dysregulation Underlying DYT1 Dystonia. J Neurosci 41:2024-2038 (2021). PubMed: 33468570