Anti-LAMP2 抗体 [GL2A7] (ab13524)
![Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.co.jp/ps/products/13/ab13524/Images/ab13524-397579-AntiLAMP2-antibody-GL2A7-western-blot-nih3t3-hek293-human-mouse.jpg "Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)")
Key features and details
- Rat monoclonal [GL2A7] to LAMP2
- Suitable for: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rabbit, Human
- Isotype: IgG
製品の概要
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製品名
Anti-LAMP2 antibody [GL2A7]
LAMP2 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [GL2A7] to LAMP2 -
由来種
Rat -
アプリケーション
適用あり: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rabbit, Human -
免疫原
Tissue, cells or virus corresponding to Mouse LAMP2. Purified preparation of mouse liver lysosomal membranes.
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ポジティブ･コントロール
- WB: HEK-293 and NIH/3T3 cell lysate.
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特記事項
For maximum product recovery, after thawing, centrifuge the product vial before removing cap.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
GL2A7 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13524の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| ICC |
Use at an assay dependent concentration.
Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
See Abreview. |
| IHC-FoFr | (1) |
Use at an assay dependent concentration.
(PubMed:17425404) |
| IP |
Use at an assay dependent concentration.
(PMID 2142158). |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (7) |
1/500.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
|
ICC/IF
Use at an assay dependent concentration. See Abreview. |
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IHC-FoFr
Use at an assay dependent concentration. (PubMed:17425404) |
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IP
Use at an assay dependent concentration. (PMID 2142158). |
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Flow Cyt
Use at an assay dependent concentration. ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/500. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. -
組織特異性
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver. -
関連疾患
Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. -
細胞内局在
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3920 Human
- Entrez Gene: 16784 Mouse
- Omim: 309060 Human
- SwissProt: P13473 Human
- SwissProt: P17047 Mouse
- Unigene: 496684 Human
- Unigene: 486 Mouse
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製品の状態
Alternative splicing produces 3 isoforms. -
別名
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
see all
画像
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Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)All lanes : Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/500 dilution (1 hr at RT)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg
Secondary
All lanes : HRP Goat Anti-Rat, 1 hour at RT at 1/100 dilutionBlock: 5% milk + TBST for 1 hour at RT.
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![Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.co.jp/ps/products/13/ab13524/Images/ab13524-260757-anti-lamp2-antibody-gl2a7-western-blot.jpg)
Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)This image is courtesy of an anonymous Abreview.Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg
Secondary
Goat anti-rat HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
Exposure time: 3 minutesBlocked with 5% milk for 1 hour at RT.
Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (207)
ab13524 は 207 報の論文で使用されています。
- Zhang X et al. Use of acidic nanoparticles to rescue macrophage lysosomal dysfunction in atherosclerosis. Autophagy 19:886-903 (2023). PubMed: 35982578
- Park K et al. Impaired TFEB activation and mitophagy as a cause of PPP3/calcineurin inhibitor-induced pancreatic β-cell dysfunction. Autophagy 19:1444-1458 (2023). PubMed: 36217215
- Walter DM et al. Setd2 inactivation sensitizes lung adenocarcinoma to inhibitors of oxidative respiration and mTORC1 signaling. Commun Biol 6:255 (2023). PubMed: 36899051
- Kumar S et al. pH regulates hematopoietic stem cell potential via polyamines. EMBO Rep 24:e55373 (2023). PubMed: 36943011
- Zhang J et al. Analgesic and potentiated photothermal therapy with ropivacaine-loaded hydrogels. Theranostics 13:2226-2240 (2023). PubMed: 37153743