Anti-LAMP1 抗体 [LY1C6] (ab13523)
Key features and details
- Mouse monoclonal [LY1C6] to LAMP1
- Suitable for: ICC, WB
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-LAMP1 antibody [LY1C6]
LAMP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [LY1C6] to LAMP1 -
由来種
Mouse -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Tissue, cells or virus corresponding to Rat LAMP1. Rat liver lysosomal membrane preparations
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ポジティブ・コントロール
- Highly purified rat lysosomal membrane preparations.
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特記事項
For maximum product recovery centrifuge the product vial before removing cap.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
LY1C6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13523の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC | (1) |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa.
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特記事項 |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa. |
ターゲット情報
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機能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
細胞内局在
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3916 Human
- Entrez Gene: 25328 Rat
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P14562 Rat
- Unigene: 494419 Human
- Unigene: 40177 Rat
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別名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
画像
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Anti-LAMP1 antibody [LY1C6] (ab13523) at 1/1000 dilution + Rat liver microsome lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP -
ab13523 at 1/100 dilution staining LAMP1 in LAMP1 transfected HeLa cells by Immunocytochemistry/ immunofluorescence. A Alexa Fluor® 568 conjugated goat anti mouse was used at 1/50 dilution as secondary.
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Western blot - Anti-LAMP1 antibody [LY1C6] (ab13523)Image from Banno A et al, J Biol Chem. 2012 Apr 20;287(17):13799-812. Epub 2012 Feb 18, Fig 4. DOI 10.1074/jbc.M112.341214 April 20, 2012 The Journal of Biological Chemistry, 287, 13799-13812.ab13523 used in Western Blot. Lysates prepared from CHO cells stably expressing aIIbß3 integrins (A5 cells) were used.
WCL, N/IC, C, P, and PM indicate whole cell lysates, nuclear/intact cell, cytosolic, high-speed pellet, and plasma membrane fractions, respectively.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab13523 は 27 報の論文で使用されています。
- Zheng P et al. Around-the-Clock Noise Induces AD-like Neuropathology by Disrupting Autophagy Flux Homeostasis. Cells 11:N/A (2022). PubMed: 36078149
- Gatault P et al. Rat and human cytomegalovirus ORF116 encodes a virion envelope glycoprotein required for infectivity. Virology 557:23-33 (2021). PubMed: 33601113
- Low SW et al. Development and characterization of a monoclonal antibody blocking human TRPM4 channel. Sci Rep 11:10411 (2021). PubMed: 34002002
- Liu X et al. PHLPP Sensitizes Multiple Myeloma Cells to Bortezomib Through Regulating LAMP2. Onco Targets Ther 13:401-411 (2020). PubMed: 32021285
- Huang S et al. Autophagy is involved in the protective effect of p21 on LPS-induced cardiac dysfunction. Cell Death Dis 11:554 (2020). PubMed: 32694519