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リコンビナントRabMAb

Anti-Laminin gamma 1 抗体 [EPR21200] - BSA and Azide free (ab234959)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)
  • Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21200] to Laminin gamma 1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free
    Laminin gamma 1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR21200] to Laminin gamma 1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human meningioma tissue.

  • 特記事項

    ab234959 is the carrier-free version of ab233390.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab234959の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 178 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 178 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

  • 組織特異性

    Found in the basement membranes (major component).

  • 配列類似性

    Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.

  • ドメイン

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human kidney (PMID: 21903675) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233390).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)

      Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human skin (PMID: 15159456) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233390).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)

      Immunohistochemical analysis of paraffin-embedded human meningioma tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in human meningioma (PMID: 23053286) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233390).

    • Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)
      Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (ab234959)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab234959 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab234959 は論文での使用が確認できていません。

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