Anti-Laminin beta 1 抗体 (ab69633)
Key features and details
- Rabbit polyclonal to Laminin beta 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Laminin beta 1 antibody
Laminin beta 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Laminin beta 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthesized peptide derived from the C-terminus of human Laminin beta 1.
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ポジティブ･コントロール
- Hela cells, human liver carcinoma tissue and extracts from HepG2
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69633の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 60, 67, 110, 130, >170 kDa (predicted molecular weight: 198 kDa).
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 60, 67, 110, 130, >170 kDa (predicted molecular weight: 198 kDa). |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
配列類似性
Contains 13 laminin EGF-like domains.
Contains 1 laminin IV type B domain.
Contains 1 laminin N-terminal domain. -
ドメイン
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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参照データベース
- Entrez Gene: 3912 Human
- Entrez Gene: 16777 Mouse
- Omim: 150240 Human
- SwissProt: P07942 Human
- SwissProt: P02469 Mouse
- Unigene: 650585 Human
- Unigene: 172674 Mouse
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別名
- CLM antibody
- Cutis laxa with marfanoid phenotype antibody
- LAM B1 antibody
see all
画像
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Western blot - Anti-Laminin beta 1 antibody (ab69633)All lanes : Anti-Laminin beta 1 antibody (ab69633) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 198 kDa
Observed band size: >170 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 130 kDa, 60 kDa, 67 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 1 antibody (ab69633)Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69633 at 1:50 dilution.
Left image un-treated.Right image treated with immunizing peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody (ab69633)Immunofluorescence analysis of HeLa cells using ab69633 at a 1:500 dilution.
Left image un-treated.Right image treated with immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab69633 は 2 報の論文で使用されています。
- El Zein RM et al. Retinoic acid receptor a as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling. Sci Rep 9:14677 (2019). PubMed: 31605007
- Cheng YC et al. Candidate tumor suppressor B-cell translocation gene 3 impedes neoplastic progression by suppression of AKT. Cell Death Dis 6:e1584 (2015). PubMed: 25569101