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AB69633

Anti-Laminin beta 1 抗体

Anti-Laminin beta 1 antibody

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(2 Publications)

Rabbit Polyclonal LAMB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LAMB1.

別名を表示する

Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 1 antibody (AB69633)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 1 antibody (AB69633)

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69633 at 1 : 50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody (AB69633)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody (AB69633)

Immunofluorescence analysis of HeLa cells using ab69633 at a 1 : 500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

Western blot - Anti-Laminin beta 1 antibody (AB69633)
  • WB

Unknown

Western blot - Anti-Laminin beta 1 antibody (AB69633)

All lanes:

Western blot - Anti-Laminin beta 1 antibody (ab69633) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells at 5 µg

Lane 2:

Extracts from HepG2 cells at 5 µg with immunising peptide

Predicted band size: 198 kDa

Observed band size: 110 kDa,130 kDa,60 kDa,67 kDa,>170 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human LAMB1. The exact immunogen used to generate this antibody is proprietary information.

P07942

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代替製品

Primary Antibodies

AB109293

Anti-Laminin beta 1 antibody [EPR3190(2)]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Laminin beta 1 antibody [EPR3190(2)] (AB109293)

  • 0
  • 0
Primary Antibodies

AB108536

Anti-Laminin beta 1 antibody [EPR3189(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (AB108536)

  • 0
  • 0
Primary Antibodies

AB88279

Anti-Laminin beta 1 antibody [DG10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 1 antibody [DG10] (AB88279)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB233389

Anti-Laminin gamma 1 antibody [EPR21199]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Laminin gamma 1 antibody [EPR21199] (AB233389)

  • 4
  • 2
Primary Antibodies

AB11575

Anti-Laminin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

  • 5
  • 74
Primary Antibodies

AB179471

Anti-Integrin beta 1 antibody [EPR16895]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] (AB179471)

  • 5
  • 5
Primary Antibodies

AB236762

Anti-Laminin 2 alpha antibody [CL3450]

Western blot - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 1 also known as LAMB1 is a subunit of the laminin molecule an essential component of the extracellular matrix. This protein has an approximate molecular weight of 200 kDa. Laminin beta 1 is one of the three chains that form the laminin heterotrimer. It is predominantly expressed in basement membranes which provide structural support and segregate tissues. The laminin 1 (laminin-111) complex including the LAMB1 chain contributes to cell adhesion differentiation migration and plays a role in tissue development and repair.
Biological function summary

This component of the laminin molecule plays a critical role in maintaining the integrity of tissues by organizing cell architecture and mediating cellular signals. Laminin beta 1 is part of a larger laminin protein complex that interacts with a variety of cell surface receptors including integrins and dystroglycan contributing to cell communication and signal transduction. These interactions guide cell orientation and regulate vital processes such as cell movement and growth.

Pathways

Laminin beta 1 participates in the integrin signaling pathway and the PI3K-AKT signaling pathway. It interacts closely with integrin proteins such as integrin alpha-6 and integrin beta-4 during these processes. In integrin signaling the laminin molecule facilitates the binding between cells and their surrounding extracellular matrix. This connection influences cellular responses including survival and motility which are necessary for normal development and wound healing processes.

Alterations in laminin beta 1 expression are linked to conditions like muscular dystrophy and Pierson syndrome. In muscular dystrophy the dysfunction of laminin beta 1 interacts with proteins such as dystrophin in muscle cells contributing to compromised structural integrity and muscle wasting. Similarly in Pierson syndrome abnormalities in laminin beta 1 are associated with a rare genetic disorder that affects the kidneys and eyes where it implicates the protein nephrin in the glomerular basement membrane leading to serious renal dysfunction.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of the cerebral cortex (PubMed : 23472759). It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons (By similarity). Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface (By similarity). As a subunit of laminin-1 (also known as laminin-111 or EHS laminin), it is involved in the stimulation of agrin-induced receptor clustering through a MuSK-independent pathway (By similarity).
See full target information LAMB1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:14677 PubMed31605007

2019

Retinoic acid receptor α as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Rami M El Zein,Audrey H Soria,Jose Felipe Golib Dzib,Amanda J Rickard,Fabio L Fernandes-Rosa,Benoit Samson-Couterie,Isabelle Giscos-Douriez,Angélique Rocha,Marko Poglitsch,Celso E Gomez-Sanchez,Laurence Amar,Norbert B Ghyselinck,Arndt Benecke,Maria-Christina Zennaro,Sheerazed Boulkroun

Cell death & disease 6:e1584 PubMed25569101

2015

Candidate tumor suppressor B-cell translocation gene 3 impedes neoplastic progression by suppression of AKT.

Applications

Unspecified application

Species

Unspecified reactive species

Y-C Cheng,P-H Chen,H-Y Chiang,C-S Suen,M-J Hwang,T-Y Lin,H-C Yang,W-C Lin,P-L Lai,S-Y Shieh
View all publications
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