JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB11575

Anti-Laminin 抗体

Anti-Laminin antibody

5

(73 Reviews)

|

(647 Publications)

Anti-Laminin antibody (ab11575) is a rabbit polyclonal antibody detecting Laminin in IHC-P, Dot Blot. Suitable for Human, Mouse.

- Over 640 publications
- Trusted since 2004

別名を表示する

LAMA, LAMA1, Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

Immunohistochemical analysis of formalin fixed paraffin-embedded human tongue sections labeling Laminin with ab11575 at 1/30 dilution. Goat Anti-Rabbit IgG FITC conjugate was used as secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

Immunohistochemical analysis of formalin fixed paraffin-embedded human tonsil sections labeling Laminin with ab11575 at 1/50 dilution. Goat Anti-Rabbit IgG Cy3™ conjugate was used as secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)
  • IHC-P

AbReview73766****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde-fixed human brain tissue. Stained with ab11575 at 1/10000 dilution. Secondary antibody used was ab150065 at 1/250 dilution. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% Normal Donkey Serum for 19 hours at 4°C. Antigen retrieval method was heat mediated, Tris/EDTA.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion anti-laminin 1 : 200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.

Dot Blot - Anti-Laminin antibody (AB11575)
  • Dot

Supplier Data

Dot Blot - Anti-Laminin antibody (AB11575)

Immunoblotting
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane was separated on SDS-PAGE and probed with Rabbit Anti-Laminin ab11575. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase secondary antibody and a chemiluminescent substrate.
Lanes
1. antibody dilution 1/1,000
2. antibody dilution 1/2,000
3. antibody dilution 1/4,000
4. antibody dilution 1/8,000
5. antibody dilution 1/16,000
6. antibody dilution 1/32,000
7. Negative control : without primary antibody

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

IHC-P, Dot

applications

免疫原

Native Full Length Protein corresponding to Human LAMA1.

P25391

特異性

In dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p>This concentration was determined using laminin at 50 ng per dot.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Amphibian": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mammals": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

製品の詳細

Anti-Laminin antibody (ab11575) is a rabbit polyclonal antibody and is validated for use in Dot and IHC-P.

Anti-Laminin antibody (ab11575) was first used in a scientific publication in 2005 and has been cited over 647 times in peer reviewed journals. It's performance in IHC and Western Blot in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Laminin antibody (ab11575) has high sensitivity and specificity.

Anti-Laminin antibody (ab11575) has 73 independent reviews from customers.

Anti-Laminin antibody (ab11575) specifically detects Laminin (UniProt ID: Q9Y6N6; Molecular weight: 170kDa) and is sold in 125 µL selling sizes.

Laminin is a crucial glycoprotein found in the extracellular matrix, essential for the structural integrity and function of basement membranes in all animals. Composed of three distinct polypeptide chains (α, β and γ), laminin forms a cross-shaped molecule that facilitates cell adhesion, differentiation, migration and tissue maintenance. Its role in biological activity makes laminin vital for understanding cellular interactions and tissue organization. Researchers study laminin to gain insights into various physiological processes and pathological conditions, including muscular dystrophy (DMD, BMD) and kidney diseases.
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 1% BSA, 0.027% PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Laminin also known as Laminin-1 is a high-molecular weight protein with a mass of approximately 400 to 900 kDa. It belongs to the family of heterotrimeric glycoproteins found in the extracellular matrix specifically within the basement membranes. This protein includes several isoforms referred to as laminins which consist of three distinct chains: α, β and γ. Laminins contribute to the structural scaffolding by interacting with other molecules like collagen and integrins playing a significant role in tissue architecture and cellular activities such as adhesion and migration. Researchers focus on targeting laminin with laminin antibodies for experiments like laminin IHC and laminin staining to visualize and study tissue structures.
Biological function summary

Laminins serve as essential components in maintaining tissue integrity and functions. They form complexes with other matrix elements to establish basement membranes which separate epithelial cells from connective tissue. Laminins interact with cellular receptors influencing cell differentiation movement and growth. Anti-laminin tools like laminin antibodies and laminin staining techniques help identify specific isoforms and analyze their distribution in research. Scientists often utilize laminine products for such purposes including insights into how they might regulate cellular environments through these complexes.

Pathways

Laminins integrate into essential biological pathways including the PI3K/Akt and MAPK pathways. Through these pathways laminins directly influence cellular processes such as proliferation and survival. They work in tandem with proteins like integrins and collagen reinforcing the structural and signaling pathways necessary for controlling cell fate. These interactions place laminins at the center of cellular communication networks regulating various developmental and physiological processes.

Laminins have connections to several medical conditions including muscular dystrophy and certain forms of cancer. Abnormalities in laminin expression or structure can lead to defective basement membranes contributing to genetic disorders like congenital muscular dystrophy. Laminins also interact with collagen another protein involved in maintaining tissue integrity making their dysfunction a point of interest in oncological research. Scientists in laminine UK and globally use anti-laminin technologies to understand laminin's role in pathogenesis aiming to develop targeted therapies.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMA1
style
left-column

文献 (647)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:17 PubMed38183022

2024

SB431542 partially inhibits high glucose-induced EMT by restoring mitochondrial homeostasis in RPE cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Cao,Mingfei Jiao,Zhenyu Kou,Feifei Han,Lijie Dong

Nature communications 14:7619 PubMed37993434

2023

Mid-old cells are a potential target for anti-aging interventions in the elderly.

Applications

Unspecified application

Species

Unspecified reactive species

Young Hwa Kim,Young-Kyoung Lee,Soon Sang Park,So Hyun Park,So Yeong Eom,Young-Sam Lee,Wonhee John Lee,Juhee Jang,Daeha Seo,Hee Young Kang,Jin Cheol Kim,Su Bin Lim,Gyesoon Yoon,Hong Seok Kim,Jang-Hee Kim,Tae Jun Park

PloS one 18:e0294395 PubMed37992082

2023

Fine-needle percutaneous muscle microbiopsy technique as a feasible tool to address histological analysis in young children with cerebral palsy and age-matched typically developing children.

Applications

Unspecified application

Species

Unspecified reactive species

Jorieke Deschrevel,Karen Maes,Anke Andries,Nathalie De Beukelaer,Marlies Corvelyn,Domiziana Costamagna,Anja Van Campenhout,Eva De Wachter,Kaat Desloovere,Anouk Agten,Frank Vandenabeele,Stefaan Nijs,Ghislaine Gayan-Ramirez

Journal of tissue engineering 14:20417314231197282 PubMed38029018

2023

A bioengineered in situ ovary (ISO) supports follicle engraftment and live-births post-chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Buckenmeyer,Meena Sukhwani,Aimon Iftikhar,Alexis L Nolfi,Ziyu Xian,Srujan Dadi,Zachary W Case,Sarah R Steimer,Antonio D'Amore,Kyle E Orwig,Bryan N Brown

Cellular and molecular biology (Noisy-le-Grand, France) 69:25-29 PubMed38015546

2023

Camk2a suppresses denervated muscle atrophy by maintaining the Ca2+ homeostasis in muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Zhang,Mingjie Xia,Tianyu Zhao,Qinyang Zhang,Rulin Li,Lei Yang

Nature communications 14:6911 PubMed37903797

2023

Microglia-mediated demyelination protects against CD8 T cell-driven axon degeneration in mice carrying PLP defects.

Applications

Unspecified application

Species

Unspecified reactive species

Janos Groh,Tassnim Abdelwahab,Yogita Kattimani,Michaela Hörner,Silke Loserth,Viktoria Gudi,Robert Adalbert,Fabian Imdahl,Antoine-Emmanuel Saliba,Michael Coleman,Martin Stangel,Mikael Simons,Rudolf Martini

Phytotherapy research : PTR 37:5916-5931 PubMed37767771

2023

Resveratrol prevents Drp1-mediated mitochondrial fission in the diabetic kidney through the PDE4D/PKA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Zhu,Zongli Deng,Yanjuan Cao,Zihui Zhou,Wen Sun,Chang Liu,Siwen Fan,Xiao-Xing Yin

Nature methods 20:1573-1580 PubMed37723243

2023

GelMap: intrinsic calibration and deformation mapping for expansion microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo G J Damstra,Josiah B Passmore,Albert K Serweta,Ioannis Koutlas,Mithila Burute,Frank J Meye,Anna Akhmanova,Lukas C Kapitein

Nature materials 22:1409-1420 PubMed37709930

2023

The laminin-keratin link shields the nucleus from mechanical deformation and signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Zanetta Kechagia,Pablo Sáez,Manuel Gómez-González,Brenda Canales,Srivatsava Viswanadha,Martín Zamarbide,Ion Andreu,Thijs Koorman,Amy E M Beedle,Alberto Elosegui-Artola,Patrick W B Derksen,Xavier Trepat,Marino Arroyo,Pere Roca-Cusachs

Nature communications 14:5665 PubMed37704631

2023

MYC Deregulation and PTEN Loss Model Tumor and Stromal Heterogeneity of Aggressive Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zinab O Doha,Xiaoyan Wang,Nicholas L Calistri,Jennifer Eng,Colin J Daniel,Luke Ternes,Eun Na Kim,Carl Pelz,Michael Munks,Courtney Betts,Sunjong Kwon,Elmar Bucher,Xi Li,Trent Waugh,Zuzana Tatarova,Dylan Blumberg,Aaron Ko,Nell Kirchberger,Jennifer A Pietenpol,Melinda E Sanders,Ellen M Langer,Mu-Shui Dai,Gordon Mills,Koei Chin,Young Hwan Chang,Lisa M Coussens,Joe W Gray,Laura M Heiser,Rosalie C Sears
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB234429

Anti-Laminin gamma 3 antibody [EPR22699-151]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Laminin gamma 3 antibody [EPR22699-151] (AB234429)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB236640

Anti-Collagen IV antibody [EPR22911-127]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com