Anti-Laminin alpha 5/LAMA5 抗体 [EPR18919] (ab184330)
![Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)](https://www.abcam.co.jp/ps/products/184/ab184330/Images/ab184330-266344-anti-laminin-alpha-5-antibody-epr18919-western-blot.jpg "Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-Laminin alpha 5/LAMA5 antibody [EPR18919]
Laminin alpha 5/LAMA5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 -
由来種
Rabbit -
特異性
This antibody reacts weakly with Laminin alpha 3 -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse Laminin alpha 5/LAMA5 and alpha 3 fragment recombinant proteins; Human fetal kidney lysate; A549 whole cell lysate; Mouse P5 fetal kidney and P3 fetal lung lysates; Rat kidney lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18919 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184330の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 404 kDa (predicted molecular weight: 404 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 404 kDa (predicted molecular weight: 404 kDa). |
ターゲット情報
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機能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
組織特異性
Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. -
配列類似性
Contains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
ドメイン
Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. -
細胞内局在
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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参照データベース
- Entrez Gene: 3911 Human
- Entrez Gene: 16776 Mouse
- Entrez Gene: 140433 Rat
- Omim: 601033 Human
- SwissProt: O15230 Human
- SwissProt: Q61001 Mouse
- Unigene: 473256 Human
- Unigene: 4339 Mouse
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別名
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
see all
画像
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Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution
Lane 1 : Mouse Laminin alpha 5/LAMA5 fragment recombinant protein
Lane 2 : Mouse Laminin alpha 3 fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 404 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes.
Mouse Laminin alpha 5/LAMA5 fragment recombinant protein contains aa46-304 with a His-Tag; Mouse Laminin alpha 3 fragment recombinant protein contains aa43-295 with a His-Tag.
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![Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)](https://www.abcam.co.jp/ps/products/184/ab184330/Images/ab184330-266343-anti-laminin-alpha-5-antibody-epr18919-western-blot.jpg)
Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 404 kDa
Observed band size: 404 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).
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![Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)](https://www.abcam.co.jp/ps/products/184/ab184330/Images/ab184330-266342-anti-laminin-alpha-5-antibody-epr18919-western-blot.jpg)
Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution
Lane 1 : Mouse P5 fetal kidney lysate
Lane 2 : Mouse P3 fetal lung lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 404 kDa
Observed band size: 404 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 30 seconds; Lane 3 and 4: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).
Negative control: mouse liver (PMID:7499364).
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![Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)](https://www.abcam.co.jp/ps/products/184/ab184330/Images/ab184330-4-benefits-of-recombinant-antibodies.png)
Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab184330 は 4 報の論文で使用されています。
- Li L et al. Ibrutinib reverses IL-6-induced osimertinib resistance through inhibition of Laminin α5/FAK signaling. Commun Biol 5:155 (2022). PubMed: 35197546
- Yang ZS et al. Regulation and Function of Laminin A5 during Mouse and Human Decidualization. Int J Mol Sci 23:N/A (2021). PubMed: 35008625
- Wu X et al. NDP-MSH binding melanocortin-1 receptor ameliorates neuroinflammation and BBB disruption through CREB/Nr4a1/NF-?B pathway after intracerebral hemorrhage in mice. J Neuroinflammation 16:192 (2019). PubMed: 31660977
- Zhang XM et al. Down-Regulation of Laminin (LN)- a5 is Associated with Preeclampsia and Impairs Trophoblast Cell Viability and Invasiveness Through PI3K Signaling Pathway. Cell Physiol Biochem 51:2030-2040 (2018). PubMed: 30522103