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Anti-Laminin alpha 5/LAMA5 抗体 [4C7] (ab17107)

Reviews (2)Q&A (3)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (ab17107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (ab17107)

Key features and details

  • Mouse monoclonal [4C7] to Laminin alpha 5/LAMA5
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Laminin alpha 5/LAMA5 antibody [4C7]
    Laminin alpha 5/LAMA5 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4C7] to Laminin alpha 5/LAMA5

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Full length native protein (purified) corresponding to Human Laminin alpha 5/LAMA5.
    Database link: O15230

  • ポジティブ･コントロール

    • Skin

  • 特記事項

    This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17107の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (2)
1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
特記事項
IHC-P
1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

  • 組織特異性

    Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.

  • 配列類似性

    Contains 22 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.

  • ドメイン

    Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer.

  • 細胞内局在

    Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
  • Information by UniProt

  • 参照データベース

    • 別名

      • KIAA0533 antibody
      • KIAA1907 antibody
      • LAMA5 antibody
      • LAMA5_HUMAN antibody
      • Laminin alpha 5 chain antibody
      • Laminin subunit alpha 5 antibody
      • Laminin subunit alpha-5 antibody
      • Laminin, alpha 5 antibody
      • Laminin-10 subunit alpha antibody
      • Laminin-11 subunit alpha antibody
      • Laminin-15 subunit alpha antibody
      • RP11-157P1.6 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (ab17107)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (ab17107)This image is courtesy of an anonymous Abreview

      ab17107 at 1/25 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. An HRP conjugated goat anti-mouse antibody was used as the secondary.

      This image was generated using the ascites version of the product.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (ab17107)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [4C7] (ab17107)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal carcinoma tissue, staining Laminin alpha 5/LAMA5 with ab17107.

      This image was generated using the ascites version of the product.

    参考文献 (2)

    ab17107 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab17107 は 2 報の論文で使用されています。

    • Ke FY  et al. Novel monoclonal antibody against integrin a3 shows therapeutic potential for ovarian cancer. Cancer Sci 111:3478-3492 (2020). PubMed: 32648337
    • Mackey AL  et al. Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. FASEB J 25:1943-59 (2011). IHC-Fr ; Human . PubMed: 21368102

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin alpha 5/LAMA5 antibody [4C7]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin explants)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Skin explants
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 03 2023

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin alpha 5/LAMA5 antibody [4C7]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Citric Acid Based (Vector Lab)
    Permeabilization
    No
    Specification
    colon
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 03 2007

    Question

    Dear Technical Team,

    Hope this e-mail finds you well!

    Please find below a complaint from one of our customerto whom we suppliedthese Abcam antibodies.

    The technical formis alsoattached in this mail.

    Request you to kindly look into it & adviseus accordingly todo further needful on it.

    Looking forward towards your valued reply/ comments on it.

    Thankingyou in advance.

    With Best Regards,

    Read More
    Answer

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    The antibody ab5654 is not tested in IHC-P application so Abpromise guarantee will not apply to this product. This antibody was however tested in WB so the guarantee will apply to WB only.

    I also have read the details you have kindly provided and have following further questions for better understanding of the problem;

    - What is the purchase order number? The number NL/Abcam 2698(N) does not match to any info we have in system.
    - Are all the three product failed?
    - What were the storage conditions of products?
    - Did you observe non specific staining, no staining or low signal?
    - Could you provide the species and type of tissues sections? e.g. human lungs
    - How the sections were fixed?
    - What sort of antigen retrieval was used? e.g. microwave with citrate buffer pH 6.0.
    - Have you tried any permeabilization step?
    - Did you try blocking the tissue sections?
    - What was the species and conjugate of secondary antibodies? Have you used these successfully before?
    - Did you try any sort of troubleshooting?
    - Could you provide the images?

    In order to get the result soon please provide all the requested details .Any additional information will also be helpful.

    I will look forward to hearing from you soon.

    Read More

    Question

    Hi, I don't want the detailed amino acid sequence about the epitope. Would you just please tell me the epitope locate in the middle or C terminal of laminin A? It will be suitable for my experiment if the epitope come from C terminal. Thanks again.

    Read More
    Answer

    Thank you for your email. Our source for this antibody has kindly provided me with the following information. In the study by Engvall, 1990, it is stated: "4C7, binds to the globular domain at the end of the long arm (Engvall et al., 1986). This domain is the C-terminal portion of the A chain and the 4C7 antibody could therefore be against the A chain." Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. Cell Regul 1:731-40 (1990). PubMed: 2099832 If you have any additional questions, please contact us again.

    Read More

    Question

    The immunogen is stated as being "The A chain of human laminin;" is the immunogen sequence available? Or, the aa range?

    Read More
    Answer

    This is the only information available to us regarding the immunogen and epitope. Further details can be found in the following two publications: Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. Cell Regul 1:731-40 (1990). PubMed: 2099832 Kleinman HK et al. Laminin receptors for neurite formation. Proc Natl Acad Sci U S A 85:1282-6 (1988). PubMed: 2963341 If you have any additional questions, please contact us again.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com