AB210957

Anti-Laminin alpha 5/LAMA5 抗体 [CL3118]

Name: Anti-Laminin alpha 5/LAMA5 抗体 [CL3118] (ab210957) | Abcam
Brand: Abcam Limited
SKU: AB210957
Price: 58000 JPY
Availability: InStock

Anti-Laminin alpha 5/LAMA5 antibody [CL3118]

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(2 Publications)

Mouse Monoclonal Laminin alpha 5/LAMA5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LAMA5 aa 2550 to C-terminus.

別名を表示する

KIAA0533, KIAA1907, LAMA5, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human lymph nodes tissue (negative control), labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human cervix tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human heart tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Supplier Data

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

All lanes:

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957) at 1/500 dilution

Lane 1:

Recombinant protein fragment of Human Laminin 511

Lane 2:

Recombinant protein fragment of Human Laminin 521

Lane 3:

Recombinant protein fragment of Human Laminin 121

Lane 4:

Recombinant protein fragment of Human Laminin 221

Lane 5:

Recombinant protein fragment of Human Laminin 332

Lane 6:

Recombinant protein fragment of Human Laminin 411

Predicted band size: 400 kDa

true

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CL3118

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human LAMA5 aa 2550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15230

エピトープ

ab210957 binds to an epitope located within the peptide sequence RQGLVDRAQQ (aa 2555 - 2564).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 5 (LAMA5) also known as laminin alpha-5 chain is an important component of the basement membrane which provides structural support to tissues. LAMA5 is a subunit of the laminin protein a large heterotrimeric glycoprotein complex involved in numerous cellular processes. The molecular weight of laminin alpha 5 can reach approximately 400 kDa. This protein is expressed widely across various tissues including the kidney lung and skin where it plays a mechanical role by mediating cell adhesion differentiation and migration by forming a scaffold-like structure at the cellular level.

Biological function summary

Laminin alpha 5 participates in the assembly of the laminin-511 complex which is vital for tissue-specific functions and maintaining cellular structures. This complex is important for epithelial and endothelial cell stability ensuring cohesive interactions with integrins and other cellular receptors. Laminin alpha 5 as part of this complex promotes development wound healing and angiogenesis impacting cell survival and regeneration by providing essential biochemical signals through its binding sites.

Pathways

Laminin alpha 5 significantly influences the PI3K/AKT and MAPK pathways which are critical for cell proliferation and survival. Within these pathways LAMA5 interacts with integrin receptors facilitating signal transduction. These pathways further associate laminin alpha 5 with proteins like FAK and Src kinase which mediate downstream signaling events influencing cellular growth and adhesion. These pathways link laminin alpha 5 to diverse cellular functions and regulatory processes.

Laminin alpha 5 has implications in diseases such as renal fibrosis and epidermolysis bullosa. Aberrant expression or mutations of LAMA5 can lead to defective basement membrane integrity affecting tissue stability and function. In renal fibrosis alterations in LAMA5 associate with disrupted kidney architecture while in epidermolysis bullosa laminin alpha 5 mutations lead to skin fragility. These conditions can also involve interactions with proteins like collagen IV and integrins contributing to the disease pathology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 (PubMed : 33242826).

See full target information LAMA5

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:7902-7914 PubMed38709270

2024

Improvement of skin wound healing by giant salamander skin mucus gel wrapped with bone marrow mesenchymal stem cells via affecting integrin family molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Dayong Du,Yan Huang,Cui Xu,Yang Liu,Xiaohong Wu,Jie Yang,Zhipeng Liu,Jianxin Ma,Chaoji Huangfu

Clinical kidney journal 17:sfae037 PubMed38455522

2024

Characterization of glomerular basement membrane components within pediatric glomerular diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Chen,Xindi Zhou,Chun Gan,Qing Yang,Wanbing Chen,Xiaoqian Feng,Tao Zhang,Li Zhang,Lujun Dai,Yaxi Chen,Haiping Yang,Mo Wang,Wei Jiang,Qiu Li

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin alpha 5/LAMA5 抗体 [CL3118]

Anti-Laminin alpha 5/LAMA5 antibody [CL3118]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise