Anti-Lamin B1 抗体

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Immunohistochemical analysis of paraffin-embedded section of human colon cancer tissue stained for Lamin B1 with ab65986 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Immunohistochemical analysis of paraffin-embedded section of human lung cancer tissue stained for Lamin B1 with ab65986 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Immunohistochemical analysis of paraffin-embedded section of human breast cancer tissue stained for Lamin B1 with ab65986 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Immunofluorescence analysis of Lamin B1 using Anti-Lamin B1 antibody - Nuclear Envelope Marker. Lamin B1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL ab65986 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Immunofluorescence analysis of Lamin B1 using Anti-Lamin B1 antibody - Nuclear Envelope Marker. Lamin B1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL ab65986 overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• IP

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Immunoprecipitation - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Immunoprecipitation of Lamin B1 in Hela whole cell lysate. Western blot analysis of Lamin B1 using Anti-Lamin B1 antibody - Nuclear Envelope Marker; Lane 1 : Hela whole cell lysates (30ug); Lane 2 : Rabbit control IgG instead of ab65986 in Hela whole cell lysate; Lane 3 : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986) (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with ab65986 at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal was developed using ECL Plus Western Blotting Substrate. A specific band was detected for Lamin B1 at approximately 70-72 kDa. The expected band size for Lamin B1 is at 66 kDa. The band at ~50kDa is IgG Heavy Chain.

All lanes:

Immunoprecipitation - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986)

All lanes:

Hela whole cell lysates

Predicted band size: 66 kDa

Observed band size: 70-72 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Immunohistochemical analysis of paraffin-embedded section of rat brain cancer tissue stained for Lamin B1 with ab65986 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Immunohistochemical analysis of paraffin-embedded section of mouse brain cancer tissue stained for Lamin B1 with ab65986 at a 2 μg/ml.

Antigen retrieval : EDTA buffer (pH 8.0, epitope retrieval solution).

• WB

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Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986)

Western blot analysis of Lamin B1 using Anti-Lamin B1 antibody - Nuclear Envelope Marker.

Blocked with 5% non-fat milk/TBS.

The signal was developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system.

All lanes:

Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986) at 0.5 µg/mL

Lane 1:

Human Hela whole cell lysates at 30 µg

Lane 2:

Human K562 whole cell lysates at 30 µg

Lane 3:

Human SH-SY5Y whole cell lysates at 30 µg

Lane 4:

Rat spleen tissue lysates, at 30 µg

Lane 5:

Rat PC-12 whole cell lysates at 30 µg

Lane 6:

Mouse RAW264.7 whole cell lysates at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP

Predicted band size: 66 kDa

Observed band size: 70-72 kDa

false