Anti-Lamin B1 抗体 (ab65986)
Key features and details
- Rabbit polyclonal to Lamin B1
- Suitable for: IHC-Fr, WB, IHC-P, ICC
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Lamin B1 antibody
Lamin B1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Lamin B1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, WB, IHC-P, ICCmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Lamin B1 aa 570-586 (C terminal).
Sequence:FHQQGTPRASNRSCAIM
Database link: P20700 -
ポジティブ・コントロール
- WB: Rat brain tissue lysate IHC-P:Mammary cancer sections; IHC-F: Rat Intestine Tissue ICC: A549 Cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65986の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml. Formalin or acetone fixed tissues.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC |
Use a concentration of 0.5 - 1 µg/ml. Acetone fixed.
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特記事項 |
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IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. Formalin or acetone fixed tissues. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa). |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC
Use a concentration of 0.5 - 1 µg/ml. Acetone fixed. |
ターゲット情報
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機能
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
関連疾患
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
細胞内局在
Nucleus inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4001 Human
- Entrez Gene: 116685 Rat
- Omim: 150340 Human
- SwissProt: P20700 Human
- SwissProt: P70615 Rat
- Unigene: 89497 Human
- Unigene: 11362 Rat
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別名
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
see all
画像
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All lanes : Anti-Lamin B1 antibody (ab65986)
Lane 1 : MCF-7 Cell lysate
Lane 2 : HeLa Cell lysate
Predicted band size: 66 kDa -
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Immunohistochemistry (Frozen sections) of Rat Intestine Tissue staining Lamin B1 with ab65986
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Immunocytochemistry of A549 Cells staining Lamin B1 with ab65986.
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Anti-Lamin B1 antibody (ab65986) at 1 µg/ml + rat brain tissue lysate
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (47)
ab65986 は 47 報の論文で使用されています。
- Zhu Y et al. Polynucleotide phosphorylase protects against renal tubular injury via blocking mt-dsRNA-PKR-eIF2α axis. Nat Commun 14:1223 (2023). PubMed: 36869030
- Jia YS et al. Beta-catenin knockdown impairs the viability of ovarian cancer cells by modulating YAP-dependent glycolysis. Am J Transl Res 15:982-994 (2023). PubMed: 36915794
- Mata-Garrido J et al. HP1γ Prevents Activation of the cGAS/STING Pathway by Preserving Nuclear Envelope and Genomic Integrity in Colon Adenocarcinoma Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37108510
- Banskota S et al. Inhibition of NADPH Oxidase (NOX) 2 Mitigates Colitis in Mice with Impaired Macrophage AMPK Function. Biomedicines 11:N/A (2023). PubMed: 37239114
- Israelsen IME et al. Cycle-dependent sex differences in expression of membrane proteins involved in cerebrospinal fluid secretion at rat choroid plexus. BMC Neurosci 24:60 (2023). PubMed: 37946101