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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Lamin B Receptor/LBR 抗体 [E398L] (ab32535)

Reviews (3) Submit a question References (50)
Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
  • Flow Cytometry (Intracellular) - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
  • Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
  • Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E398L] to Lamin B Receptor/LBR
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-Lamin B Receptor/LBR antibody [E398L]
    Lamin B Receptor/LBR 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [E398L] to Lamin B Receptor/LBR

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide within Human Lamin B Receptor/LBR. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • IF: HeLa cells. WB: HEK-293 and Jurkat whole cell lysate (ab7899). Flow Cyt (intra): HeLa cells. IHC-P: Human breast carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32535の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/500. Detects a band of approximately 67 kDa (predicted molecular weight: 71 kDa).
ICC/IF (1)
1/500.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/500. Detects a band of approximately 67 kDa (predicted molecular weight: 71 kDa).
ICC/IF
1/500.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Anchors the lamina and the heterochromatin to the inner nuclear membrane.

  • 関連疾患

    Defects in LBR are a cause of Pelger-Huet anomaly (PHA) [MIM:169400]. PHA is an autosomal dominant inherited abnormality of neutrophils, characterized by reduced nuclear segmentation and an apparently looser chromatin structure. Heterozygotes show hypolobulated neutrophil nuclei with coarse chromatin. Presumed homozygous individuals have ovoid neutrophil nuclei, as well as varying degrees of developmental delay, epilepsy, and skeletal abnormalities.
    Defects in LBR are the cause of hydrops-ectopic calcification-moth-eaten skeletal dysplasia (HEM) [MIM:215140]; also known as Greenberg skeletal dysplasia. HEM is a rare autosomal recessive chondrodystrophy characterized by early in utero lethality and, therefore, considered to be nonviable. Affected fetuses typically present with fetal hydrops, short-limbed dwarfism, and a marked disorganization of chondro-osseous calcification and may present with polydactyly and additional nonskeletal malformations.
    Defects in LBR may be a cause of Reynolds syndrome (REYNS) [MIM:613471]. It is a syndrome specifically associating limited cutaneous systemic sclerosis and primary biliray cirrhosis. It is characterized by liver disease, telangiectasia, abrupt onset of digital paleness or cyanosis in response to cold exposure or stress (Raynaud phenomenon), and variable features of scleroderma. The liver disease is characterized by pruritis, jaundice, hepatomegaly, increased serum alkaline phosphatase and positive serum mitochondrial autoantibodies, all consistent with primary biliary cirrhosis.

  • 配列類似性

    Belongs to the ERG4/ERG24 family.

  • 翻訳後修飾

    Phosphorylated by CDK1 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.

  • 細胞内局在

    Nucleus inner membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DHCR 14B antibody
      • DHCR14B antibody
      • Integral nuclear envelope inner membrane protein antibody
      • Lamin-B receptor antibody
      • LBR antibody
      • LBR_HUMAN antibody
      • LMN 2R antibody
      • LMN2R antibody
      • MGC9041 antibody
      • PHA antibody
      • PRO0650 antibody
      see all

    画像

    • Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      All lanes : Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535) at 1/500 dilution

      Lane 1 : Wild-type HEK-293 cell lysate
      Lane 2 : Human LBR (Lamin B Receptor) knockout HEK-293T cell lysate (ab257503)

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 71 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab32535 observed at 58 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab32535 was shown to react with Lamin B Receptor/LBR in wild-type HEK-293 cells in western blot. Loss of signal was observed when LBR knockout lysate ab257503 was used. Wild-type and LBR knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab32535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

      Ab32535, at a 1/500 dilution, staining Lamin B Receptor/LBR in HeLa cells by Immunofluorescence.

    • Flow Cytometry (Intracellular) - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      Flow Cytometry (Intracellular) - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Lamin B Receptor/LBR with unpurified ab32535 at 1/20 dilutionc (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      IHC image of ab32535 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32535, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535) at 1/500 dilution + Jurkat cell lysate

      Predicted band size: 71 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?

    • Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
      Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (50)

    ab32535 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32535 は 50 報の論文で使用されています。

    • Wu X  et al. Triptolide promotes ferroptosis by suppressing Nrf2 to overcome leukemia cell resistance to doxorubicin. Mol Med Rep 27:N/A (2023). PubMed: 36453238
    • Liu Y  et al. Systematic analysis of integrated bioinformatics to identify upregulated THBS2 expression in colorectal cancer cells inhibiting tumour immunity through the HIF1A/Lactic Acid/GPR132 pathway. Cancer Cell Int 23:253 (2023). PubMed: 37884956
    • Xu Y  et al. Paeoniflorin inhibits proliferation and promotes autophagy and apoptosis of sweat gland cells. Exp Ther Med 23:53 (2022). PubMed: 34934430
    • Tsai PL  et al. Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins. Cell Rep 41:111675 (2022). PubMed: 36417855
    • Zhang Z  et al. METTL14 Regulates Intestine Cellular Senescence through m6A Modification of Lamin B Receptor. Oxid Med Cell Longev 2022:9096436 (2022). PubMed: 36578521
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    ChIP abreview for Anti-Lamin B Receptor/LBR antibody [E398L]

     Good
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (K562)
    Specification
    K562
    Detection step
    Other
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Andrew Perez

    Verified customer

    投稿 Mar 01 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B Receptor/LBR antibody [E398L]

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse embryonic fibroblasts)
    Permeabilization
    Yes - 0.5% triton X
    Specification
    Mouse embryonic fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 12 2017

    Western blot abreview for Anti-Lamin B Receptor/LBR antibody [E398L]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (NCI-H929)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    200000 cells
    Specification
    NCI-H929
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 20 2012

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