AB8984

Anti-Lamin A + Lamin C 抗体 [131C3] - Nuclear Envelope Marker

Name: Anti-Lamin A + Lamin C 抗体 [131C3] - Nuclear Envelope Marker (ab8984)
Brand: Abcam Limited
SKU: AB8984
Price: 56000 JPY
Availability: InStock
Rating: 4 (12 reviews)

Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker

Lab Essentials
詳細を見る

(12 Reviews)

(94 Publications)

Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984) is a mouse monoclonal antibody detecting Lamin A + Lamin C in Flow Cytometry, IHC-Fr. Suitable for Human, Pig.

- Over 80 publications
- Trusted since 2002

別名を表示する

LMN1, LMNA, Prelamin-A/C

4 Images

Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

IHC-Fr of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

ab8984 on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.

Flow Cytometry - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

Flow Cyt

Unknown

Flow Cytometry - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

Overlay histogram showing HeLa cells stained with ab8984 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8984, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

ab8984 (1/500) on frozen sections of pig skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

131C3

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

交差種

Human, Pig

アプリケーション

Flow Cyt, IHC-Fr

applications

免疫原

Native Full Length Protein corresponding to Rat Lmna.

P48679

エピトープ

Between residues 319-566.

特異性

ab8984 reacts with lamins A and C. Lamins do not appear to be universally distributed among different cell and tissue types. ab8984 has been shown to react with HT1080 cells in Western blot. Other cell/tissue types have not been tested. Customer feedback seems to indicate that ab8984 does not detect Lamin A+C in rat brain lysates (please see AbReviews).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/200", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "Recommended range given is for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent." }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "Recommended range given is for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent." } } }

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製品の詳細

What is this antibody validated in?
Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (ab8984) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-Fr) in Human, Pig samples.

Trusted by the scientific community
Anti-Lamin A + Lamin C [131C3] - Nuclear Envelope Marker (ab8984) was first used in a scientific publication in 2002 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-Lamin A + Lamin C [131C3] - Nuclear Envelope Marker (ab8984) has over 10 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

Preservative: 0.09% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.

Biological function summary

Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.

Pathways

Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.

Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Lamin-A/C. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 2188730, PubMed : 22431096, PubMed : 2344612, PubMed : 23666920, PubMed : 24741066, PubMed : 31434876, PubMed : 31548606, PubMed : 37788673, PubMed : 37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 22431096, PubMed : 23666920, PubMed : 24741066, PubMed : 31548606, PubMed : 37788673, PubMed : 37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed : 23990565, PubMed : 25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 2188730, PubMed : 2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 22431096, PubMed : 23666920, PubMed : 31548606). Also involved in DNA repair : recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed : 31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed : 10080180, PubMed : 10814726, PubMed : 11799477, PubMed : 18551513, PubMed : 22431096). Required for osteoblastogenesis and bone formation (PubMed : 12075506, PubMed : 15317753, PubMed : 18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed : 10587585). Required for cardiac homeostasis (PubMed : 10580070, PubMed : 12927431, PubMed : 18611980, PubMed : 23666920).. Prelamin-A/C. Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed : 20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed : 20458013).

See full target information LMNA

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文献 (94)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1725-1738 PubMed41034521

2025

Microtubule architecture connects AMOT stability to YAP/TAZ mechanotransduction and Hippo signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Giada Vanni,Anna Citron,Ambela Suli,Paolo Contessotto,Robin Caire,Alessandro Gandin,Giovanna Mantovan,Francesca Zanconato,Giovanna Brusatin,Michele Di Palma,Elisa Peirano,Lisa Sofia Pozzer,Carlo Albanese,Roberto A Steiner,Michelangelo Cordenonsi,Tito Panciera,Stefano Piccolo

Bioactive materials 48:100-117 PubMed40034808

2025

Integrated biomimetic bioprinting of perichondrium with cartilage for auricle reconstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Litao Jia,Siyu Liu,Luosha Gu,Xiaomin Liu,Kexin Sun,Feiyang Chu,Jinshi Zeng,Wenshuai Liu,Haiyue Jiang,Xia Liu

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39060175

2024

Calmodulin Triggers Activity-Dependent rRNA Biogenesis via Interaction with DDX21.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Lin Yang,Xue Sun,Jun-Xiu Shi,Qing-Xu Cui,Xin-Yu Cao,Kai-Tuo Wang,Ming-Xin An,Si-Jin Wu,Yong-Liang Yang,Hong-Zan Sun,Wei-Dong Zhao

Scientific reports 14:13727 PubMed38877142

2024

Loss of connectin novex-3 leads to heart dysfunction associated with impaired cardiomyocyte proliferation and abnormal nuclear mechanics.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Hashimoto,Momoko Ohira,Aya Kodama,Misaki Kimoto,Mariko Inoue,Shigenobu Toné,Yuu Usui,Akira Hanashima,Takato Goto,Yuhei Ogura,Yoshihiro Ujihara,Satoshi Mohri

Nature cardiovascular research 3:441-459 PubMed38765203

2024

Histone H1.0 couples cellular mechanical behaviors to chromatin structure.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaishuai Hu,Douglas J Chapski,Natalie D Gehred,Todd H Kimball,Tatiana Gromova,Angelina Flores,Amy C Rowat,Junjie Chen,René R Sevag Packard,Emily Olszewski,Jennifer Davis,Christoph D Rau,Timothy A McKinsey,Manuel Rosa-Garrido,Thomas M Vondriska

Life science alliance 7: PubMed38228373

2024

The LINC complex ensures accurate centrosome positioning during prophase.

Applications

Unspecified application

Species

Unspecified reactive species

Joana T Lima,António J Pereira,Jorge G Ferreira

Nucleic acids research 52:1930-1952 PubMed38109320

2023

Nuclear localization of Argonaute 2 is affected by cell density and may relieve repression by microRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Krystal C Johnson,Audrius Kilikevicius,Cristina Hofman,Jiaxin Hu,Yang Liu,Selina Aguilar,Jon Graswich,Yi Han,Tao Wang,Jill M Westcott,Rolf A Brekken,Lan Peng,Georgios Karagkounis,David R Corey

Cell proliferation 57:e13566 PubMed37864298

2023

PML-mediated nuclear loosening permits immunomodulation of mesenchymal stem/stromal cells under inflammatory conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Yunpeng Chu,Zishan Jiang,Zheng Gong,Xiaocao Ji,Mengting Zhu,Qianwen Shang,Pixia Gong,Lijuan Cao,Yongjing Chen,Peishan Li,Changshun Shao,Yufang Shi

Nature materials 22:1409-1420 PubMed37709930

2023

The laminin-keratin link shields the nucleus from mechanical deformation and signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Zanetta Kechagia,Pablo Sáez,Manuel Gómez-González,Brenda Canales,Srivatsava Viswanadha,Martín Zamarbide,Ion Andreu,Thijs Koorman,Amy E M Beedle,Alberto Elosegui-Artola,Patrick W B Derksen,Xavier Trepat,Marino Arroyo,Pere Roca-Cusachs

Nature communications 14:3867 PubMed37391402

2023

Nuclear lamina strain states revealed by intermolecular force biosensor.

Applications

Unspecified application

Species

Unspecified reactive species

Brooke E Danielsson,Bobin George Abraham,Elina Mäntylä,Jolene I Cabe,Carl R Mayer,Anna Rekonen,Frans Ek,Daniel E Conway,Teemu O Ihalainen

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Lamin A + Lamin C 抗体 [131C3] - Nuclear Envelope Marker

Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker

Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

Flow Cytometry - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

Immunohistochemistry (Frozen sections) - Anti-Lamin A + Lamin C antibody [131C3] - Nuclear Envelope Marker (AB8984)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (94)

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