Anti-Lamin A + Lamin B1 + Lamin C 抗体 [EPR4068]
Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
- 20ul selling size
- RabMAb
- Recombinant
- Lab Essentials
- 詳細を見る
5
(6 Reviews)
|
(44 Publications)
Rabbit Recombinant Monoclonal Lamin B1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 44 publications.
別名を表示する
LMN2, LMNB, LMNB1, Lamin-B1, LMN1, LMNA, Prelamin-A/C, LMN1, LMNA, Prelamin-A/C
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Unpurified ab108922 showing positive staining in Normal human kidney tissue.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Immunohistochemical analysis of paraffin-embedded human colonic tissue using unpurified ab108922 at a diltion of 1/100
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Intracellular Flow Cytometry analysis of HeLa cells labelling Lamin A + B1 + C with purified ab108922 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Immunofluorescence staining of HeLa cells with purified ab108922 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108922 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Unpurified ab108922 showing positive staining in Normal human breast tissue.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Immunohistochemical staining of paraffin embedded human liver carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Unpurified ab108922 showing positive staining in Normal human uterus tissue.
- IP
Unknown
Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
ab108922 (purified) at 1/20 immunoprecipitating Lamin A + B1 + C in 10 μg HepG2 (Lanes 1 and 2, observed at 70, 74, and 65 kDa - ab108922 recognises three isoforms). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration : 5% NFDM/TBST.
Dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
false
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Immunohistochemical staining of paraffin embedded rat liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Immunohistochemical staining of paraffin embedded mouse liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- WB
Lab
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/10000 dilution
All lanes:
HeLa cell lysate at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution
Observed band size: 65 kDa,70 kDa,74 kDa
false
- WB
Unknown
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
All lanes:
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution
Lane 1:
HeLa cell lysate at 10 µg
Lane 2:
HepG2 cell lysate at 10 µg
Lane 3:
HACAT cell lysate at 10 µg
false
- WB
Lab
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/10000 dilution
Lane 1:
mouse heart lysate at 10 µg
Lane 2:
rat heart lysate at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution
Predicted band size: 130 kDa,30 kDa,47 kDa
Observed band size: 65 kDa,70 kDa,74 kDa
false
- WB
Lab
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Blocking/Diluting buffer and concentration : 5% NFDM/TBST.
ab108922 does not cross react with Lamin B2.
Lane 1:
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution
Lane 2:
Anti-Myc tag antibody at 1/10000 dilution
Lane 3:
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/5000 dilution
All lanes:
Myc and DDK tagged recombinant Human Lamin B2 protein at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 68 kDa
false
Exposure time: 7s
- WB
Lab
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)
Blocking/Diluting buffer and concentration 5% NFDM/TBST
All lanes:
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution
Lane 1:
Western blot - Recombinant Human Lamin A protein (<a href='/products/proteins-peptides/recombinant-human-lamin-a-protein-ab83472'>ab83472</a>) at 10 µg
Lane 2:
GST tagged recombinant Human Lamin B1 protein (1 to 586) (91 KDa) at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 75 kDa,91 kDa
false
Exposure time: 180s
関連する標識済み抗体及び組成の異なる製品 (10)
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Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - BSA and Azide free
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660 APC
APC Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
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HRP Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
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578 PE
PE Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
Reactivity data
製品の詳細
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Lamin proteins regulate nuclear architecture and maintain chromatin organization. They participate in forming the nuclear lamina a dense fibrillar network inside the nucleus. The lamina provides structural support to the nuclear envelope and plays roles in DNA replication and transcription. Lamin molecules also interact with nuclear pore complexes therefore contributing to molecular transport across the nuclear envelope. Lamin A/C and Lamin B1 function together with other proteins like emerin and lamin associated polypeptides in forming multi-protein complexes that influence cell cycle regulation and differentiation.
Pathways
Lamin proteins integrate into pathways governing the cell cycle and DNA repair. They contribute to the mitotic pathway assisting in the disassembly and reassembly of the nuclear envelope during cell division. Lamin molecules also engage in the DNA damage response pathway cooperating with repair proteins such as p53 and BRCA1. Through these pathways lamin proteins ensure proper progression through the cell cycle and maintain genomic stability by aiding in the repair of DNA damage.
製品プロトコール
- Visit the General protocols
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文献 (44)
Recent publications for all applications. Explore the full list and refine your search
Biochemistry and biophysics reports 44:102241 PubMed40978197
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Cells 12: PubMed37443716
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Nature neuroscience 26:737-750 PubMed37095396
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British journal of pharmacology 180:1056-1071 PubMed36440573
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iScience 25:105352 PubMed36325059
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EMBO reports 23:e55251 PubMed36214648
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Abcam product promise
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