Name: Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]
Brand: RabMAb
SKU: ab108922
Rating: 5 (6 reviews)

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Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

製品名

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]
Lamin A+Lamin B1+Lamin C 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
由来種

Rabbit
特異性

The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.
アプリケーション

適用あり: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Human
交差が予測される動物種: Spermophilus tridecemlineatus
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Mouse heart and rat heart tissue lysates and HeLa, HepG2 and HACAT cell lysates, ab83472. IHC-P: Human ovarian carcinoma, human liver carcinoma, human breast, human uterus, human kidney mouse liver and rat liver tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HepG2 lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR4068
アイソタイプ

IgG
研究分野
- Cell Biology
- Apoptosis
- Nucleus
- Lamins
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Lamins

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab108922の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF		1/100 - 1/250.
Flow Cyt (Intra)		1/20.
WB	(4)	1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
IP		1/10 - 1/100.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

特記事項
ICC/IF 1/100 - 1/250.
Flow Cyt (Intra) 1/20.
WB 1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

細胞内局在

Lamin A: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C. Lamin B1: Nucleus inner membrane. Lamin C: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C.
参照データベース
- Entrez Gene: 4000 Human
- Entrez Gene: 4001 Human
- Entrez Gene: 16905 Mouse
- Entrez Gene: 16906 Mouse
- Entrez Gene: 116685 Rat
- Entrez Gene: 60374 Rat
- Omim: 150330 Human
- Omim: 150340 Human
- SwissProt: P02545 Human
- SwissProt: P02545-2 Human
- SwissProt: P20700 Human
- SwissProt: P14733 Mouse
- SwissProt: P48678 Mouse
- SwissProt: P48679 Rat
- SwissProt: P70615 Rat
- Unigene: 594444 Human
- Unigene: 89497 Human
- Unigene: 243014 Mouse
- Unigene: 4105 Mouse
- Unigene: 471227 Mouse
- Unigene: 11362 Rat
- Unigene: 44161 Rat
see all
製品の状態

Lamin C: There are 3 isoforms produced by alternative splicing. Isoform A also known as Lamin A; Isoform C also known as Lamin C; Isoform ADelta10 also known as Lamin ADelta10.

Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/10000 dilution (purified)

Lane 1 : mouse heart lysate
Lane 2 : rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 74 kDa
Additional bands at: 65 kDa (possible isoform), 70 kDa (possible isoform), 74 kDa (possible isoform)

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunofluorescence staining of HeLa cells with purified ab108922 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108922 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical staining of paraffin embedded rat liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution

Lane 1 : Recombinant Human Lamin A protein (ab83472)
Lane 2 : GST tagged recombinant Human Lamin B1 protein (1 to 586) (91 KDa)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 75, 91 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking/Diluting buffer and concentration 5% NFDM/TBST
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Lane 1 : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution
Lane 2 : Anti-Myc tag antibody at 1/10000 dilution
Lane 3 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/5000 dilution

All lanes : Myc and DDK tagged recombinant Human Lamin B2 protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?

Exposure time: 7 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

ab108922 does not cross react with Lamin B2.
Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Intracellular Flow Cytometry analysis of HeLa cells labelling Lamin A + B1 + C with purified ab108922 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour^®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

ab108922 (purified) at 1/20 immunoprecipitating Lamin A + B1 + C in 10 μg HepG2 (Lanes 1 and 2, observed at 70, 74, and 65 kDa - ab108922 recognises three isoforms). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.

Dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 74 kDa
Additional bands at: 65 kDa (possible isoform), 70 kDa (possible isoform), 74 kDa (possible isoform)

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical staining of paraffin embedded human liver carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical staining of paraffin embedded mouse liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical analysis of paraffin-embedded human colonic tissue using unpurified ab108922 at a diltion of 1/100
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HACAT cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 74 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Unpurified ab108922 showing positive staining in Normal human breast tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Unpurified ab108922 showing positive staining in Normal human uterus tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Unpurified ab108922 showing positive staining in Normal human kidney tissue.
Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Datasheet download

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ab108922 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108922 は 34 報の論文で使用されています。

Udeochu JC et al. Tau activation of microglial cGAS-IFN reduces MEF2C-mediated cognitive resilience. Nat Neurosci 26:737-750 (2023). PubMed: 37095396
Liao W et al. Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function. Br J Pharmacol 180:1056-1071 (2023). PubMed: 36440573
Kober P et al. Epigenetic Downregulation of Hsa-miR-193b-3p Increases Cyclin D1 Expression Level and Cell Proliferation in Human Meningiomas. Int J Mol Sci 24:N/A (2023). PubMed: 37686289
Chua HH et al. PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis. Cell Mol Gastroenterol Hepatol 15:121-152 (2023). PubMed: 36191855
Yang R et al. Premature aging of skeletal stem/progenitor cells rather than osteoblasts causes bone loss with decreased mechanosensation. Bone Res 11:35 (2023). PubMed: 37407584

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-6 of 6 Abreviews or Q&A

Application

ChIP

Sample

Human Cell lysate - nuclear (K562)

Negative control

IgG

Specification

K562

Detection step

Other

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Andrew Perez

Verified customer

投稿 Mar 24 2022

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Macrophage Cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

Macrophage Cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 17 2018

Application

Flow Cytometry

Sample

Mouse Cell (Mouse embryonic fibroblasts (wild type and LMNA kn)

Permeabilization

Yes - 0.1% saponin

Specification

Mouse embryonic fibroblasts (wild type and LMNA kn

Fixation

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 18 2017

Application

Western blot

Sample

Human Cell lysate - nuclear (HEK293 Lysate (kidney cell))

Gel Running Conditions

Reduced Denaturing (4-12% Gradient)

Loading amount

5 µg

Specification

HEK293 Lysate (kidney cell)

Blocking step

Licor Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Sam Nowitzki

Verified customer

投稿 Jul 28 2015

Application

Western blot

Sample

Human Cell lysate - nuclear (HEK nuclear and cytoplasmic lysates)

Gel Running Conditions

Reduced Denaturing

Loading amount

15 µg

Specification

HEK nuclear and cytoplasmic lysates

Blocking step

Licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 08 2015

Application

Western blot

Sample

Spermophilus tridecemlineatus Cell lysate - nuclear (Brown adipose tissue)

Gel Running Conditions

Reduced Denaturing (10% Tris-Glycine)

Loading amount

30 µg

Specification

Brown adipose tissue

Blocking step

BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. James Bjork

Verified customer

投稿 Dec 19 2012

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

細胞内局在

参照データベース

製品の状態

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (34)

レビューと Q&A

ChIP abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Western blot abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Flow Cytometry abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Western blot abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Western blot abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Western blot abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]