Anti-Lambda Free Light Chain 抗体 [3D12] (ab1944)
Key features and details
- Mouse monoclonal [3D12] to Lambda Free Light Chain
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
製品の概要
-
製品名
Anti-Lambda Free Light Chain antibody [3D12] -
製品の詳細
Mouse monoclonal [3D12] to Lambda Free Light Chain -
由来種
Mouse -
特異性
Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Lambda Free Light Chain.
-
ポジティブ・コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
-
特記事項
Concentration varies from lot to lot and can be provided on request.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Protein A purified -
特記事項(精製)
Purity tested by electrophoresis. -
ポリ/モノ
モノクローナル -
クローン名
3D12 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1944の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 5 µg/ml.
|
特記事項 |
---|
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
-
関連性
standard
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)
IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab1944 は論文での使用が確認できていません。