AB209238

Anti-LAG-3 抗体 [EPR20294-77]

Name: Anti-LAG-3 抗体 [EPR20294-77] (ab209238) | Abcam
Brand: Abcam Limited
SKU: AB209238
Price: 26000 JPY
Availability: InStock
Rating: 5 (4 reviews)

Anti-LAG-3 antibody [EPR20294-77]

RabMAb
Recombinant
20ul selling size
詳細を見る

(4 Reviews)

(8 Publications)

Anti-LAG-3 antibody [EPR20294-77] (ab209238) is a rabbit monoclonal antibody detecting LAG-3 in Western Blot, IP, ICC/IF. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

CD223, Lymphocyte activation gene 3 protein, LAG-3, Activation-induced cytidine deaminase-linked autoimmunity protein, Aida, Lag3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized untreated and concanavalin A (2.5 μg/ml for 72 hours) treated mouse splenocytes labeling LAG-3 with ab209238 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing increased membranous staining following treatment of mouse splenocytes with concanavalin A (2.5 μg/ml for 72 hours) (PMID 20391435; PMID 26318293; PMID 11527700; PMID 19631993).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution

Supplier Data

Immunoprecipitation - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

LAG-3 was immunoprecipitated from 0.35 mg of mouse splenocytes (treated with 5 μg/ml concanavalin A for 72 hours) whole cell lysate with ab209238 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse splenocytes (treated with 5 μg/ml concanavalin A for 72 hours), whole cell lysate 10μg (Input).

Lane 2 : ab209238 IP in mouse splenocytes (treated with 5 μg/ml concanavalin A for 72 hours), whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab209238 in mouse splenocytes (treated with 5 μg/ml concanavalin A for 72 hours), whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-LAG-3 antibody [EPR20294-77] (ab209238)

Predicted band size: 57 kDa

Observed band size: 54 kDa,70 kDa

false

Supplier Data

Western blot - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

Blocking/Dilution Buffer : 5% NFDM/TBST

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

LAG3 expression can be elevated in activated splenocytes (PMID 19631993).

The molecular mass observed is consistent with the literature (PMID 15971272; PMID 15557174).

All lanes:

Western blot - Anti-LAG-3 antibody [EPR20294-77] (ab209238) at 1/1000 dilution

Lane 1:

Untreated mouse splenocytes whole cell lysate at 10 µg

Lane 2:

Mouse splenocytes treated with 5 ug/ml concanavalin A for 72 hours whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 54 kDa,70 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

Blocking/Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-LAG-3 antibody [EPR20294-77] (ab209238) at 1/10000 dilution

All lanes:

Mouse LAG-3 recombinant protein (aa24-442) tagged with murine IgG2A at the C-terminus, 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 100 kDa

true

Exposure time: 1s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20294-77

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

ICC/IF, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-LAG-3 antibody [EPR20294-77] (ab209238) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

What is the molecular weight of LAG-3?
Anti-LAG-3 [EPR20294-77] (ab209238) specifically detects a band for LAG-3 (UniProt: Q61790) at a molecular weight of 57kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20294-77] also available for your convenience: ab209238, Carrier free - ab236154

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LAG-3 also known as CD223 is a transmembrane protein with a mass of approximately 70 kDa. It acts as a checkpoint molecule similar to CTLA-4 and PD-1 in the immune system. LAG-3 is expressed on activated T cells natural killer cells and some dendritic cells. It interacts with its ligand MHC class II leading to inhibition of cellular proliferation and cytokine secretion an important part of immune response regulation.

Biological function summary

LAG-3 is involved in maintaining immune homeostasis and preventing autoimmunity. It is not part of a complex but works in synergy with other immune checkpoint molecules. When interacting with MHC class II LAG-3 transmits inhibitory signals that attenuate immune cell responses. Its role in immune regulation helps to balance immune activation and tolerance which is essential for preventing tissue damage while protecting against pathogens.

Pathways

The inhibitory role of LAG-3 integrates into the T cell receptor (TCR) signaling pathway and is also linked to the PD-1 signaling pathway. In TCR signaling LAG-3 negatively regulates T cell activation. LAG-3 is functionally related to proteins like PD-1 and CTLA-4 sharing similar roles in immune checkpoint pathways that are critical for maintaining immune balance.

LAG-3 has links to cancer and autoimmune diseases. In cancer LAG-3 expression on tumor-infiltrating lymphocytes correlates with immune evasion by tumors. This relationship appears in various cancers including melanoma and renal cell carcinoma. In autoimmune diseases LAG-3's expression on regulatory T cells helps control conditions such as multiple sclerosis. It collaborates with CTLA-4 in modulating immune responses that prevent excessive immune activation and tissue damage associated with autoimmunity.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Lymphocyte activation gene 3 protein : Inhibitory receptor on antigen activated T-cells (PubMed : 12209638, PubMed : 12421911, PubMed : 12672063, PubMed : 15100286, PubMed : 15634887, PubMed : 30580966). Delivers inhibitory signals upon binding to ligands, such as FGL1 (PubMed : 30580966). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (PubMed : 30580966). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed : 12209638, PubMed : 12421911, PubMed : 12672063, PubMed : 15100286, PubMed : 15634887). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (PubMed : 21300912). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed : 12209638, PubMed : 12421911, PubMed : 12672063, PubMed : 15100286, PubMed : 15634887). Also mediates immune tolerance : constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (PubMed : 15485628). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (PubMed : 19201841). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed : 12209638, PubMed : 12421911, PubMed : 15634887).. Secreted lymphocyte activation gene 3 protein. May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.

See full target information Lag3

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40067762

2025

Propionyl-CoA carboxylase subunit B regulates anti-tumor T cells in a pancreatic cancer mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Han V Han,Richard Efem,Barbara Rosati,Kevin Lu,Sara Maimouni,Ya-Ping Jiang,Valeria Montoya,Ando Van Der Velden,Wei-Xing Zong,Richard Z Lin

Cancer discovery 15:162-178 PubMed39348506

2024

Combined KRAS Inhibition and Immune Therapy Generates Durable Complete Responses in an Autochthonous PDAC Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghong Liu,Jincheng Han,Wen-Hao Hsu,Kyle A LaBella,Pingna Deng,Xiaoying Shang,Paulino Tallón de Lara,Li Cai,Shan Jiang,Ronald A DePinho

Frontiers in cellular neuroscience 17:1308972 PubMed38026700

2023

LAG-3 expression in microglia regulated by IFN-γ/STAT1 pathway and metalloproteases.

Applications

Unspecified application

Species

Unspecified reactive species

Yuta Morisaki,Motoki Ohshima,Hikaru Suzuki,Hidemi Misawa

iScience 26:106698 PubMed37182098

2023

Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed oligodendrocytes in mutant mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tassnim Abdelwahab,David Stadler,Konrad Knöpper,Panagiota Arampatzi,Antoine-Emmanuel Saliba,Wolfgang Kastenmüller,Rudolf Martini,Janos Groh

Nature neuroscience 25:1446-1457 PubMed36280798

2022

CD8 T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging.

Applications

Unspecified application

Species

Unspecified reactive species

Tuğberk Kaya,Nicola Mattugini,Lu Liu,Hao Ji,Ludovico Cantuti-Castelvetri,Jianping Wu,Martina Schifferer,Janos Groh,Rudolf Martini,Simon Besson-Girard,Seiji Kaji,Arthur Liesz,Ozgun Gokce,Mikael Simons

The Journal of international medical research 50:3000605221126874 PubMed36173010

2022

Fibrinogen like protein-1 knockdown suppresses the proliferation and metastasis of TU-686 cells and sensitizes laryngeal cancer to LAG-3 blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Jiameng Huang,Qiang Huang,Jiyao Xue,Huiqin Liu,Yang Guo,Hui Chen,Liang Zhou

Biomolecules 9: PubMed31569553

2019

Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use.

Applications

Unspecified application

Species

Unspecified reactive species

Quentin Lecocq,Katty Zeven,Yannick De Vlaeminck,Sandrina Martens,Sam Massa,Cleo Goyvaerts,Geert Raes,Marleen Keyaerts,Karine Breckpot,Nick Devoogdt

Cell reports 27:1277-1292.e7 PubMed31018140

2019

Transcriptomic Hallmarks of Tumor Plasticity and Stromal Interactions in Brain Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Wingrove,Zongzhi Z Liu,Kiran D Patel,Anna Arnal-Estapé,Wesley L Cai,Mary-Ann Melnick,Katerina Politi,Cátia Monteiro,Lucía Zhu,Manuel Valiente,Harriet M Kluger,Veronica L Chiang,Don X Nguyen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAG-3 抗体 [EPR20294-77]

Anti-LAG-3 antibody [EPR20294-77]

Immunocytochemistry/ Immunofluorescence - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

Immunoprecipitation - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

Western blot - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

Western blot - Anti-LAG-3 antibody [EPR20294-77] (AB209238)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

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Abcam product promise