Anti-L3MBTL3 抗体 (ab68117)
Key features and details
- Mouse polyclonal to L3MBTL3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-L3MBTL3 antibody
L3MBTL3 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to L3MBTL3 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human L3MBTL3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68117の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa). |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Required for normal maturation of myeloid progenitor cells. -
配列類似性
Contains 3 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 84456 Human
- SwissProt: Q96JM7 Human
- Unigene: 658051 Human
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別名
- L3MBTL3 antibody
- H-l(3)mbt-like protein 3 antibody
- Hl(3)mbt like protein antibody
see all
画像
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Western blot - Anti-L3MBTL3 antibody (ab68117)All lanes : Anti-L3MBTL3 antibody (ab68117) at 1/500 dilution
Lane 1 : L3MBTL3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Predicted band size: 88 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL3 antibody (ab68117)Immunofluorescence analysis of HeLa cells using purified ab68117 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab68117 は 1 報の論文で使用されています。
- Wang M et al. L3MBTL3 is induced by HIF-1α and fine tunes the HIF-1α degradation under hypoxia in vitro. Heliyon 9:e13222 (2023). PubMed: 36747531
