AB9440

Anti-L Glutamate 抗体

Anti-L Glutamate antibody

JP Deleterious:医薬用外劇物

(5 Reviews)

(16 Publications)

Rabbit Polyclonal L Glutamate antibody. Suitable for IHC-Fr, IHC-FoFr and reacts with Chemical samples. Cited in 16 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to L Glutamate.

別名を表示する

L glutamate

1 Images

IHC-Fr

AbReview37126****

Immunohistochemistry (Frozen sections) - Anti-L Glutamate antibody (AB9440)

ab9440 staining L Gluramate in Mouse retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol, permeabilized with 0.025% TBS-Triton X-100 and blocked with 5% serum for 1 hour. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Patricia Bogdanov

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

アプリケーション

IHC-Fr, IHC-FoFr

applications

特異性

The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against glutamate in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, D/L-aspartate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) was detected against D-glutamate. Significant cross-reactivity (1:8) was detected against free NAAG in competition assays (NAAG is not retained in aldehyde-fixed tissue).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p>Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur.</p>" } } }

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製品の詳細

The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

バッファー組成

Preservative: 0.05% Thimerosal (merthiolate) Constituents: Phosphate Buffer, 1% Goat Serum

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

L-glutamate also called glutamic acid is an important neurotransmitter in the central nervous system. This small amino acid has a molecular weight of 147.13 g/mol. Neurons and glial cells both express L-glutamate which allows it to function widely in the brain. Synaptic terminals release L-glutamate into the synaptic cleft where it binds to glutamate receptors on the postsynaptic neuron to propagate neural signals.

Biological function summary

L-glutamate serves key roles in synaptic plasticity and cognitive functions including learning and memory. It acts as the main excitatory neurotransmitter in the human brain and is essential for normal brain activities. L-glutamate does not belong to a protein complex but it operates closely with other neurotransmitters and their receptors ensuring proper signal transmission between neurons.

Pathways

L-glutamate is involved in synaptic signaling and metabolic pathways like the glutamatergic synapse pathway. This amino acid interacts with proteins such as NMDA and AMPA receptors that are integral to these pathways. The proper functioning of these pathways is critical for maintaining excitatory neurotransmission and overall brain health.

Irregular L-glutamate signaling relates to conditions such as Alzheimer’s disease and epilepsy. In Alzheimer’s disease altered glutamate signaling contributes to neurodegeneration with NMDA receptor overactivation playing a role. In epilepsy excessive glutamate release leads to hyperexcitation contributing to seizures. Understanding these associations helps to identify potential therapeutic targets for treating these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information L Glutamate

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:6 PubMed39844330

2025

Glutamine-glutamate centered metabolism as the potential therapeutic target against Japanese encephalitis virus-induced encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyuan Li,Hang Yuan,Xiaofei Yang,Yingfeng Lei,Jianqi Lian

Vision (Basel, Switzerland) 8: PubMed38804347

2024

Biological Sunglasses in a Deep-Sea Squid: Pigment Migration in the Retina of .

Applications

Unspecified application

Species

Unspecified reactive species

Ryan B Howard,Jessica Kniller,Kathrin S R Bolstad,Monica L Acosta

Molecular vision 29:274-288 PubMed38222448

2024

Changes in glutamate and glutamine distributions in the retinas of cystine/glutamate antiporter knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Luis J Knight,Renita M Martis,Paul J Donaldson,Monica L Acosta,Julie C Lim

Biomaterials research 26:84 PubMed36539898

2022

A functional neuron maturation device provides convenient application on microelectrode array for neural network measurement.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Han,Naoki Matsuda,Yuto Ishibashi,Aoi Odawara,Sayuri Takahashi,Norie Tooi,Koshi Kinoshita,Ikuro Suzuki

Frontiers in neuroscience 15:805070 PubMed34938159

2021

Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jing Yu,Tong Xiao,Xiao-Jing Liu,Ying Li,Jie Qi,Nianping Zhang,Li-Yan Fu,Kai-Li Liu,Yanjun Li,Yu-Ming Kang

International journal of molecular sciences 22: PubMed34502087

2021

Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at -25 °C for Several Years.

Applications

Unspecified application

Species

Unspecified reactive species

Akito Otubo,Sho Maejima,Takumi Oti,Keita Satoh,Yasumasa Ueda,John F Morris,Tatsuya Sakamoto,Hirotaka Sakamoto

Disease models & mechanisms 13: PubMed32680850

2020

Loss of the branched-chain α-ketoacid dehydrogenase complex results in neuronal dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Ying Tsai,Shih-Cheng Wu,Jian-Chiuan Li,Yu-Min Chen,Chih-Chiang Chan,Chun-Hong Chen

Molecular vision 26:277-290 PubMed32300272

2020

Proinflammatory cytokines trigger biochemical and neurochemical changes in mouse retinal explants exposed to hyperglycemic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Gaganashree Shivashankar,Julie C Lim,Monica L Acosta

Frontiers in neuroscience 14:161 PubMed32256305

2020

Evidence of Synaptic and Neurochemical Remodeling in the Retina of Aging Degus.

Applications

Unspecified application

Species

Unspecified reactive species

Lily Y-L Chang,Alvaro O Ardiles,Cheril Tapia-Rojas,Joaquin Araya,Nibaldo C Inestrosa,Adrian G Palacios,Monica L Acosta

Nature communications 5:4159 PubMed24923909

2014

Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzheimer's [corrected] disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wu,Ziyuan Guo,Marla Gearing,Gong Chen

View all publications

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Abcam product promise

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Anti-L Glutamate 抗体

Anti-L Glutamate antibody

Immunohistochemistry (Frozen sections) - Anti-L Glutamate antibody (AB9440)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

アプリケーション

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (16)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise