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Anti-Kv4.3/KCND3 抗体 (ab65794)

Reviews (1)Q&A (4)References (3)

Key features and details

  • Rabbit polyclonal to Kv4.3/KCND3
  • Suitable for: IHC-Fr, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Kv4.3/KCND3 antibody
    Kv4.3/KCND3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Kv4.3/KCND3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab65794の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.

  • 組織特異性

    Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.

  • 配列類似性

    Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily.

  • ドメイン

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

  • 細胞内局在

    Membrane. Cell membrane > sarcolemma.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • KCND 3 antibody
    • KCND 3L antibody
    • KCND 3S antibody
    • Kcnd3 antibody
    • KCND3_HUMAN antibody
    • KCND3L antibody
    • KCND3S antibody
    • KSHIVB antibody
    • Kv 4.3 antibody
    • MGC14203 antibody
    • MGC142037 antibody
    • OTTHUMP00000063948 antibody
    • Potassium ioinic channel Kv4.3 antibody
    • Potassium voltage gated channel Shal related subfamily member 3 antibody
    • Potassium voltage gated channel subfamily D member 3 antibody
    • Potassium voltage-gated channel subfamily D member 3 antibody
    • Sha1 related potassium channel Kv4.3 antibody
    • Voltage gated K+ channel antibody
    • Voltage gated potassium channel Kv4.3 antibody
    • Voltage gated potassium channel subunit Kv4.3 antibody
    • Voltage-gated potassium channel subunit Kv4.3 antibody
    see all

参考文献 (3)

ab65794 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab65794 は 3 報の論文で使用されています。

  • Ren KW  et al. Cardiac-specific knockdown of Bhlhe40 attenuates angiotensin II (Ang II)-Induced atrial fibrillation in mice. Front Cardiovasc Med 9:957903 (2022). PubMed: 36304536
  • Zhan X  et al. FMRP(1-297)-tat restores ion channel and synaptic function in a model of Fragile X syndrome. Nat Commun 11:2755 (2020). PubMed: 32488011
  • Matkovich SJ  et al. MicroRNA-133a protects against myocardial fibrosis and modulates electrical repolarization without affecting hypertrophy in pressure-overloaded adult hearts. Circ Res 106:166-75 (2010). WB ; Human . PubMed: 19893015

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Kv4.3/KCND3 antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Transfected chinese hamster ovary cells)
Gel Running Conditions
Reduced Denaturing (any KD biorad)
Loading amount
20 µg
Treatment
Harvested after 48h transfection
Specification
Transfected chinese hamster ovary cells
Blocking step
Odyssey blocking buffer PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alberto Rossetti

Verified customer

投稿 Feb 24 2021

Question

This antibody is not the only one I bought from your company. Others worked well using the same protocol and none of them had such total black imaging. All of these three were using this antibody(first time I thought maybe some mistakes involving secondary antibody, so I repeated the WB in another two gels and got the same result).  I may change the blocking and incubation buffer to BSA(actually I already used bsa in incubation buffer) or the blocking buffer you suggested. But what I am most concerned now is if this antibody is suitable for WB in rat tissues.  Any similar complaint regarding this antibody? I really don’t want to spend another two days just to repeat the same result.

Read More
Answer

I understand your concern.

We have not seen any complaints of this type with this antibody. Although I cannot guarantee that your following experiment will not yield similar results, I have seen this type of response with other product before. Usually type of response does occur as a reaction to the blocking buffer but using freshly made buffers and reagents would also be advisable. Do you pre-rinse your membrane prior to transfer with methanol?

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Read More

Question

The following is the protocol I used:             1.    Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE, 7.5%) on rat DRG ;  2.    Transfer the proteins to PVDF. 3.    Wash the blotted PVDF twice with 0.1% Tween 20 TBST. 4.     Block the blotted PVDF in freshly prepared TBST containing 5% nonfat dry milk for 60 minutes at room temperature with constant agitation. 5.     Incubate the PVDF with 1:1,000 of anti-Kv4.3 (ab65794), diluted in freshly prepared 2% TBST-BSA overnight at 4°C with agitation. 6.    Wash the PVDF 3 times with TBST for 10 minutes each. 7.    Incubate the PVDF in the secondary antibody (Goat Anti-Rabbit IgG (H+L), HRP Conjugate, 1:5000) for 60 minutes at room temperature with agitation. 8.    Wash the PVDF 3 times with TBST for 10 minutes each. 9.    Use detection method of enhanced chemiluminescence to develop imaging.   30s exposure

Read More
Answer

Thank you for providing this additional information.

It seems apparent that the antibody is reacting to something on this blot. It is usually the case in instances like this that there is reaction to the proteins in your blocking buffer. I would strongly recommend changing your blocking and incubation buffers from milk to acasein-based blocking solution or to BSA. We find that the casin based buffers give the best results in our studies and do not react with the antibodies like this. Our10X Blocking Buffer can be found at the following link:https://www.abcam.com/10X-Blocking-Buffer-ab126587.html

I would also recommend running a "no primary" control as well to ensure that it is not the secondary which is reacting with something.

Finally, I would suggest using a nitrocellulose membrane to determine if this is a reaction with the PVDF.

Please let me know how these suggestions work for you and if you have any questions or concerns.

Read More

Question

I bought an antibody(ab65794) in your company two weeks ago. This antibody didn’t work for WB of rat DRGs, which is predicted to work in rats in the datasheet. The imaging was totally black in the X ray film in the area of this antibody while other part with actin  worked normally.  I tested this antibody again in two gels and still got the same results. Could you please send me a replacement(not necessary the same one) or just refund for this product? Thanks. Looking forward to hearing from you.  

Read More
Answer

Thank you for providing information.

I am sorry to hear of the issues that you have been experiencing.In order to help with our investigation of this case before deciding how to proceed, I would appreciate if you are able to provide the details of the protocol that you have used. Any troubleshooting steps you may have taken as well as images would also be very helpful. This information will also be helpful to our quality monitoring.

If we are unable to rectify this issue and this product has been purchased within the last 6 months,I will be pleased to provide a free of charge replacement or credit note.

Thank you for your time. I look forward to hearing from you with the requested information and hope we can resolve this case as soon as possible.

Read More

Question

  Dear Mr./Ms,   We are working with the Kv4.3 protein and three month ago, we bought an antibody (ab65794) in your company. This antibody doesn't work very well for WB, IP, ICC and IHC. Now, I am trying to find a good antibody. We need a huge amount of anti-Kv4.3 and I wonder if, before we buy it again, you could send us an aliquot to check it. We are interested in you product numbers: ab115163, ab49348 and ab99045. We will appreciate your help as we do not have a good experience with this antibody.     King regards,  

Read More
Answer

Thank you for contacting Abcam. I am sorry to hear that ab65794 has not been working for you. I will be glad to help you find an alternative antibody. To help determine the antibody that will best suit your needs, could you tell me what tissue/species you are working in. Also is there any application that you are primarily interested in eg WB, IHC-P, IP, ICC. I look forward to hearing back from you and helping to find an antibody for you.

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