Anti-Kv4.2/KCND2 抗体 [EP982Y] (ab46797)
Key features and details
- Rabbit monoclonal [EP982Y] to Kv4.2/KCND2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-Kv4.2/KCND2 antibody [EP982Y]
Kv4.2/KCND2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP982Y] to Kv4.2/KCND2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human brain lysate. IHC-P: Human cerebrum tissue.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP982Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46797の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 80 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. Detects a band of approximately 80 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits. -
組織特異性
Highly expressed throughout the brain. Expression is very low or absent in other tissues. -
配列類似性
Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily. -
ドメイン
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
翻訳後修飾
Phosphorylated on serine and threonine residues. -
細胞内局在
Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression. - Information by UniProt
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参照データベース
- Entrez Gene: 3751 Human
- Omim: 605410 Human
- SwissProt: Q9NZV8 Human
- Unigene: 654739 Human
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別名
- KCD2 antibody
- KCND 2 antibody
- KCND2 antibody
see all
画像
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Anti-Kv4.2/KCND2 antibody [EP982Y] (ab46797) at 1/1000 dilution + Human brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue sections labeling Kv4.2/KCND2 with purified ab46797 at 1/100 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab46797 は 3 報の論文で使用されています。
- Korkka I et al. Heterogeneity of Potassium Channels in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium. Stem Cells Transl Med 11:753-766 (2022). PubMed: 35639962
- Cacace R et al. Loss of DPP6 in neurodegenerative dementia: a genetic player in the dysfunction of neuronal excitability. Acta Neuropathol 137:901-918 (2019). WB ; Human . PubMed: 30874922
- Aronica E et al. Expression and localization of voltage dependent potassium channel Kv4.2 in epilepsy associated focal lesions. Neurobiol Dis 36:81-95 (2009). PubMed: 19596445