Anti-Kv1.6/RCK2 抗体 (ab21089)
Key features and details
- Rabbit polyclonal to Kv1.6/RCK2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Kv1.6/RCK2 antibody -
製品の詳細
Rabbit polyclonal to Kv1.6/RCK2 -
由来種
Rabbit -
特異性
In human HEK293 cells ab21089 recognised a band of ~58 kDa, the predicted MW of Kv1.6/RCK2 (data not shown). This band was not blocked by the immunising peptide. Additional bands of 100 kDa, 25 kDa and 20 kDa were also recognised.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human Kv1.6/RCK2 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23431) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21089の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
ターゲット情報
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機能
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. -
配列類似性
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily. -
ドメイン
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3742 Human
- Entrez Gene: 16494 Mouse
- Entrez Gene: 64358 Rat
- Omim: 176257 Human
- SwissProt: P17658 Human
- SwissProt: Q61923 Mouse
- SwissProt: P17659 Rat
- Unigene: 306190 Human
see all -
別名
- HBK 2 antibody
- HBK2 antibody
- Human brain potassium channel 2 antibody
see all
画像
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Western blot - Anti-Kv1.6/RCK2 antibody (ab21089)All lanes : Anti-Kv1.6/RCK2 antibody (ab21089) at 1 µg/ml
Lane 1 : Mouse Brain Lysate
Lane 2 : Mouse Brain Lysate Kv1.6/RCK2 with Human Kv1.6/RCK2 peptide (ab23431) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lane 2 : Alexa Fluor Goat polyclonal to Rabbit IgG
Predicted band size: 58 kDaab21089 recognises a band corresponding to the predicted MW of Kv1.6/RCK2 in mouse brain lysate (lane 1). Antibody binding to this band may be blocked using the immunising peptide, ab23431 (lane 2). The antibody also recognises additional proteins. These may be degradation/cleavage products of Kv1.6/RCK2, or they may represent non-specific binding by the antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Kv1.6/RCK2 antibody (ab21089)ICC/IF image of ab21089 stained human HEK (Human embryonic kidney) 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab21089, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab21089 は 1 報の論文で使用されています。
- Suda M et al. Adult-onset hypothyroidism causes mechanical hypersensitivity due to peripheral nerve hyperexcitability based on voltage-gated potassium channel downregulation in male mice. J Neurosci Res 100:506-521 (2022). PubMed: 34935172